Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on cleft palate-related genes in mouse embryonic palatal mesenchymal cells

Tomohiro Yamada, Katsuaki Mishima, Kumiko Fujiwara, Hideto Imura, Norifumi Moritani, Toshio Sugahara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To investigate the possible involvement of 2,3,7,8- tetrachlorodibenzo-p-dioxin in the pathogenesis of cleft palate in mouse palatal cells. Materials and Methods: Palatal shelves of gestation day 12.5 ICR mouse foetuses were removed and cultured with serum. One day after the second passage, the medium was changed to serum-free and 2,3,7,8-tetrachlorodibenzo-p-dioxin was added at 1 ng/mL, 10 ng/mL, and 100 ng/mL. After culture for 1 and 3 hours, all cellular RNA was isolated from the cells. Expression of some cleft palate-related genes was analysed semiquantitatively using reverse transcriptase-polymerase chain reaction. Five genes (Tgfb3, Msxl, Lhx8, Fgfrl, and Fgfr2) were selected on the basis that functional disturbance of these genes causes cleft palate with high frequency. The data were compared with GAPDH. Results: In the first passage, many mouse embryonic palatal mesenchymal cells had spread out, and a number of monolayer spindle-shaped cells were identified. The cells were stable in the second passage and also in the serum-free medium. One hour after addition oj'2,3,7,8-tetrachlorodibenzo-p-dioxin, Tgfb3 mRNA expression was reduced at a concentration of 100 ng/mL. After 3 hours, Tgfb3, Msxl, and Lhx8 mRNA expression were also reduced. However, there was no effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the gene expression for Fgfrl and Fgfr2 mRNA. Conclusion: 2,3,7,8-Tetrachlorodibenzo-p-dioxin may contribute to the induction of cleft palate by suppressing Tgfb3, Msxl, and Lhx8 gene expression.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume18
Issue number2
Publication statusPublished - Jun 2006

Fingerprint

Cleft Palate
Genes
Messenger RNA
Gene Expression
Inbred ICR Mouse
Serum-Free Culture Media
Reverse Transcriptase Polymerase Chain Reaction
Serum
Fetus
Polychlorinated Dibenzodioxins
RNA
Pregnancy

Keywords

  • Cleft palate
  • Dioxins
  • Polymerase chain reaction
  • Tetrachlorodibenzodioxin

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine
  • Oral Surgery

Cite this

Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on cleft palate-related genes in mouse embryonic palatal mesenchymal cells. / Yamada, Tomohiro; Mishima, Katsuaki; Fujiwara, Kumiko; Imura, Hideto; Moritani, Norifumi; Sugahara, Toshio.

In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 18, No. 2, 06.2006, p. 93-98.

Research output: Contribution to journalArticle

Yamada, Tomohiro ; Mishima, Katsuaki ; Fujiwara, Kumiko ; Imura, Hideto ; Moritani, Norifumi ; Sugahara, Toshio. / Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on cleft palate-related genes in mouse embryonic palatal mesenchymal cells. In: Asian Journal of Oral and Maxillofacial Surgery. 2006 ; Vol. 18, No. 2. pp. 93-98.
@article{fd26446bbb414b50b406fc0e34b038e9,
title = "Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on cleft palate-related genes in mouse embryonic palatal mesenchymal cells",
abstract = "Objective: To investigate the possible involvement of 2,3,7,8- tetrachlorodibenzo-p-dioxin in the pathogenesis of cleft palate in mouse palatal cells. Materials and Methods: Palatal shelves of gestation day 12.5 ICR mouse foetuses were removed and cultured with serum. One day after the second passage, the medium was changed to serum-free and 2,3,7,8-tetrachlorodibenzo-p-dioxin was added at 1 ng/mL, 10 ng/mL, and 100 ng/mL. After culture for 1 and 3 hours, all cellular RNA was isolated from the cells. Expression of some cleft palate-related genes was analysed semiquantitatively using reverse transcriptase-polymerase chain reaction. Five genes (Tgfb3, Msxl, Lhx8, Fgfrl, and Fgfr2) were selected on the basis that functional disturbance of these genes causes cleft palate with high frequency. The data were compared with GAPDH. Results: In the first passage, many mouse embryonic palatal mesenchymal cells had spread out, and a number of monolayer spindle-shaped cells were identified. The cells were stable in the second passage and also in the serum-free medium. One hour after addition oj'2,3,7,8-tetrachlorodibenzo-p-dioxin, Tgfb3 mRNA expression was reduced at a concentration of 100 ng/mL. After 3 hours, Tgfb3, Msxl, and Lhx8 mRNA expression were also reduced. However, there was no effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the gene expression for Fgfrl and Fgfr2 mRNA. Conclusion: 2,3,7,8-Tetrachlorodibenzo-p-dioxin may contribute to the induction of cleft palate by suppressing Tgfb3, Msxl, and Lhx8 gene expression.",
keywords = "Cleft palate, Dioxins, Polymerase chain reaction, Tetrachlorodibenzodioxin",
author = "Tomohiro Yamada and Katsuaki Mishima and Kumiko Fujiwara and Hideto Imura and Norifumi Moritani and Toshio Sugahara",
year = "2006",
month = "6",
language = "English",
volume = "18",
pages = "93--98",
journal = "Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology",
issn = "2212-5558",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on cleft palate-related genes in mouse embryonic palatal mesenchymal cells

AU - Yamada, Tomohiro

AU - Mishima, Katsuaki

AU - Fujiwara, Kumiko

AU - Imura, Hideto

AU - Moritani, Norifumi

AU - Sugahara, Toshio

PY - 2006/6

Y1 - 2006/6

N2 - Objective: To investigate the possible involvement of 2,3,7,8- tetrachlorodibenzo-p-dioxin in the pathogenesis of cleft palate in mouse palatal cells. Materials and Methods: Palatal shelves of gestation day 12.5 ICR mouse foetuses were removed and cultured with serum. One day after the second passage, the medium was changed to serum-free and 2,3,7,8-tetrachlorodibenzo-p-dioxin was added at 1 ng/mL, 10 ng/mL, and 100 ng/mL. After culture for 1 and 3 hours, all cellular RNA was isolated from the cells. Expression of some cleft palate-related genes was analysed semiquantitatively using reverse transcriptase-polymerase chain reaction. Five genes (Tgfb3, Msxl, Lhx8, Fgfrl, and Fgfr2) were selected on the basis that functional disturbance of these genes causes cleft palate with high frequency. The data were compared with GAPDH. Results: In the first passage, many mouse embryonic palatal mesenchymal cells had spread out, and a number of monolayer spindle-shaped cells were identified. The cells were stable in the second passage and also in the serum-free medium. One hour after addition oj'2,3,7,8-tetrachlorodibenzo-p-dioxin, Tgfb3 mRNA expression was reduced at a concentration of 100 ng/mL. After 3 hours, Tgfb3, Msxl, and Lhx8 mRNA expression were also reduced. However, there was no effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the gene expression for Fgfrl and Fgfr2 mRNA. Conclusion: 2,3,7,8-Tetrachlorodibenzo-p-dioxin may contribute to the induction of cleft palate by suppressing Tgfb3, Msxl, and Lhx8 gene expression.

AB - Objective: To investigate the possible involvement of 2,3,7,8- tetrachlorodibenzo-p-dioxin in the pathogenesis of cleft palate in mouse palatal cells. Materials and Methods: Palatal shelves of gestation day 12.5 ICR mouse foetuses were removed and cultured with serum. One day after the second passage, the medium was changed to serum-free and 2,3,7,8-tetrachlorodibenzo-p-dioxin was added at 1 ng/mL, 10 ng/mL, and 100 ng/mL. After culture for 1 and 3 hours, all cellular RNA was isolated from the cells. Expression of some cleft palate-related genes was analysed semiquantitatively using reverse transcriptase-polymerase chain reaction. Five genes (Tgfb3, Msxl, Lhx8, Fgfrl, and Fgfr2) were selected on the basis that functional disturbance of these genes causes cleft palate with high frequency. The data were compared with GAPDH. Results: In the first passage, many mouse embryonic palatal mesenchymal cells had spread out, and a number of monolayer spindle-shaped cells were identified. The cells were stable in the second passage and also in the serum-free medium. One hour after addition oj'2,3,7,8-tetrachlorodibenzo-p-dioxin, Tgfb3 mRNA expression was reduced at a concentration of 100 ng/mL. After 3 hours, Tgfb3, Msxl, and Lhx8 mRNA expression were also reduced. However, there was no effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the gene expression for Fgfrl and Fgfr2 mRNA. Conclusion: 2,3,7,8-Tetrachlorodibenzo-p-dioxin may contribute to the induction of cleft palate by suppressing Tgfb3, Msxl, and Lhx8 gene expression.

KW - Cleft palate

KW - Dioxins

KW - Polymerase chain reaction

KW - Tetrachlorodibenzodioxin

UR - http://www.scopus.com/inward/record.url?scp=78650591678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650591678&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 93

EP - 98

JO - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology

JF - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology

SN - 2212-5558

IS - 2

ER -