Ectopic calcification: Importance of common nanoparticle scaffolds containing oxidized acidic lipids

Hiromi Kumon, Eiji Matsuura, Noriyuki Nagaoka, Toshio Yamamoto, Shinya Uehara, Motoo Araki, Yukana Matsunami, Kazuko Kobayashi, Akira Matsumoto

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The term nanobacteria, sometimes referred to as nanobacteria-like particles (NLPs), is presently recognized as a misnomer for inert calcified nanoparticles. However, misinterpretation of its propagation as a living organism still continues. Ultrastructural and elemental analyses, combining immuno-electron microscopy with an original NLP isolate (P-17) derived from urinary stones, and an IgM monoclonal antibody (CL-15) raised against P-17 have now revealed that, oxidized lipids with acidified functional groups were key elements in NLP propagation. Lamellar structures composed of acidic/oxidized lipids provided structural scaffolds for carbonate apatite crystals. During in vitro culture, lipid peroxidation induced by γ-irradiation of FBS was a major cause of accelerated NLP propagation. In pathological tissue samples from hyperlipidemic atherosclerosis-prone mice, CL-15 co-localized with fatty plaques, macrophage infiltrates and osteocalcin staining of aortic valve lesions. These observations indicate that naturally occurring NLP composed of mineralo-oxidized lipids complexes are generated as by-products rather than etiological agents of chronic inflammation. From the Clinical Editor: The term "nanobacteria-like particles (NLPs)" is presently recognized as a misnomer for inert calcified nanoparticles as opposed to living organisms. This study convincingly demonstrates that naturally occurring NLPs composed of mineralo-oxidized lipid complexes are generated as by-products rather than etiological agents of chronic inflammation.

Original languageEnglish
Pages (from-to)441-450
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2014

Keywords

  • Ectopic calcification
  • Nanobacteria-like particles
  • Nanoparticles
  • Oxidized lipids

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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