Ectopic accumulation of linalool confers resistance to Xanthomonas citri subsp. citri in transgenic sweet orange plants

Takehiko Shimada, Tomoko Endo, Ana Rodríguez, Hiroshi Fujii, Shingo Goto, Takakazu Matsuura, Yuko Hojo, Yoko Ikeda, Izumi C. Mori, Takashi Fujikawa, Leandro Peña, Mitsuo Omura

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    In order to clarify whether high linalool content in citrus leaves alone induces strong field resistance to citrus canker caused by Xanthomonas citri subsp. citri (Xcc), and to assess whether this trait can be transferred to a citrus type highly sensitive to the bacterium, transgenic 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) plants over-expressing a linalool synthase gene (CuSTS3-1) were generated. Transgenic lines (LIL) with the highest linalool content showed strong resistance to citrus canker when spray inoculated with the bacterium. In LIL plants inoculated by wounding (multiple-needle inoculation), the linalool level was correlated with the repression of the bacterial titer and up-regulation of defense-related genes. The exogenous application of salicylic acid, methyl jasmonate or linalool triggered responses similar to those constitutively induced in LIL plants. The linalool content in Ponkan mandarin leaves was significantly higher than that of leaves from six other representative citrus genotypes with different susceptibilities to Xcc. We propose that linalool-mediated resistance might be unique to citrus tissues accumulating large amounts of volatile organic compounds in oil cells. Linalool might act not only as a direct antibacterial agent, but also as a signal molecule involved in triggering a non-host resistance response against Xcc.

    Original languageEnglish
    Pages (from-to)654-664
    Number of pages11
    JournalTree physiology
    Volume37
    Issue number5
    DOIs
    Publication statusPublished - May 1 2017

    Keywords

    • canker
    • citrus
    • non-host resistance
    • systematic acquired resistance

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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