Easy sectioning of whole grain of rice using cryomicrotome

Wan Yi Chiou, Kazuo Tsugane, Tadafumi Kawamoto, Masahiko Maekawa

    Research output: Contribution to journalComment/debatepeer-review

    4 Citations (Scopus)

    Abstract

    To obtain a clear intact section of a ripened rice grain, which is suitable for biochemical and histological analysis, the Kawamoto method using a specific adhesive film was applied using a cryomicrotome. The longitudinal and sagittal sections were easily obtained together with the cross-section, and cell characteristics were clearly discerned in the ripened grain. It was demonstrated that the Kawamoto method is readily applicable for intact sectioning of hard tissue, including ripened grain. Intact section sampling may be useful for enzymatic analysis and transcriptomic analysis of plant tissue.

    Original languageEnglish
    Pages (from-to)381-384
    Number of pages4
    JournalBreeding Science
    Volume68
    Issue number3
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Cross section
    • Cryomicrotome
    • Easy sectioning
    • Longitudinal section
    • Matured grain
    • Rice

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Genetics
    • Plant Science

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