Early response in water relations influenced by NACL reflects tolerance or sensitivity of barley plants to salinity stress via aquaporins

Maki Katsuhara, Ji Ye Rhee, Genki Sugimoto, Gap Chae Chung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Barley varieties, K305 and I743, which are sodium chloride (NaCl) tolerant and sensitive respectively, were hydroponically grown to determine the short-term effects of NaCl on the cell water relations in roots using a cell pressure probe, and on the transcript levels of 10 barley PIP aquaporin genes (HvPIPs) in roots. Stomatal conductance, as an indicator of sensitivity to NaCl, was decreased to less than half values of control upon exposure to 100 mmol L-1 NaCl for 24h in I743 whereas tolerant variety, K305, was able to maintain original conductance. Osmotic half-times of water exchange in cortical cells allowed for a clear distinction between the two varieties up to 200 mmol L-1 NaCl. With treatment duration of up to 12h with 100 mmol L-1 NaCl, the elastic modulus was reduced in I743 but increased in K305. Hydrostatic half-times of water exchange in K305 increased rapidly, whereas this value remained unchanged in I743. Application of abscisic acid (ABA) after 1 h NaCl treatment restored the hydraulic conductivity of cells (Lp) in K305 but not in I743 whereas the opposite results were obtained when mercury chloride (HgCl2) was applied, verifying the contrasting gating response of aquaporins in two varieties. Reduced expression of HvPIPs was consistent with the reduction of hydraulic conductivity of both varieties after 24h NaCl, but without any significant differences between them, indicating the importance of the activities of existing aquaporins rather than de novo synthesis to cope with short-term effects of salt stress.

Original languageEnglish
Pages (from-to)50-60
Number of pages11
JournalSoil Science and Plant Nutrition
Volume57
Issue number1
DOIs
Publication statusPublished - Feb 2011

Fingerprint

aquaporins
water relations
water exchange
barley
sodium chloride
salt stress
hydraulic conductivity
tolerance
salinity
abscisic acid
elastic modulus
stomatal conductance
hydrostatics
water
probe
chloride
salt
gene
cells
effect

Keywords

  • Aquaporin
  • Barley
  • Elastic modulus
  • Hydraulic conductivity
  • Salinity

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Cite this

Early response in water relations influenced by NACL reflects tolerance or sensitivity of barley plants to salinity stress via aquaporins. / Katsuhara, Maki; Rhee, Ji Ye; Sugimoto, Genki; Chung, Gap Chae.

In: Soil Science and Plant Nutrition, Vol. 57, No. 1, 02.2011, p. 50-60.

Research output: Contribution to journalArticle

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AB - Barley varieties, K305 and I743, which are sodium chloride (NaCl) tolerant and sensitive respectively, were hydroponically grown to determine the short-term effects of NaCl on the cell water relations in roots using a cell pressure probe, and on the transcript levels of 10 barley PIP aquaporin genes (HvPIPs) in roots. Stomatal conductance, as an indicator of sensitivity to NaCl, was decreased to less than half values of control upon exposure to 100 mmol L-1 NaCl for 24h in I743 whereas tolerant variety, K305, was able to maintain original conductance. Osmotic half-times of water exchange in cortical cells allowed for a clear distinction between the two varieties up to 200 mmol L-1 NaCl. With treatment duration of up to 12h with 100 mmol L-1 NaCl, the elastic modulus was reduced in I743 but increased in K305. Hydrostatic half-times of water exchange in K305 increased rapidly, whereas this value remained unchanged in I743. Application of abscisic acid (ABA) after 1 h NaCl treatment restored the hydraulic conductivity of cells (Lp) in K305 but not in I743 whereas the opposite results were obtained when mercury chloride (HgCl2) was applied, verifying the contrasting gating response of aquaporins in two varieties. Reduced expression of HvPIPs was consistent with the reduction of hydraulic conductivity of both varieties after 24h NaCl, but without any significant differences between them, indicating the importance of the activities of existing aquaporins rather than de novo synthesis to cope with short-term effects of salt stress.

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