Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide

Naoko Ohara, Yoshihiko Hayashi, Shizuka Yamada, Se Kwon Kim, Tsunenori Matsunaga, Kajiro Yanagiguchi, Takeshi Ikeda

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Chitosan has a variety of biological activities. However, little is known about how chitosan modulates the hard tissue forming cells. When we cultured an osteoblastic cell line in α-MEM supplemented with 10% FBS and 0.005% chitooligosaccharide for 3 days, alkaline phosphatase (ALP) activity was significantly high compared with the control culture group (p

Original languageEnglish
Pages (from-to)1749-1754
Number of pages6
JournalBiomaterials
Volume25
Issue number10
DOIs
Publication statusPublished - May 2004
Externally publishedYes

Fingerprint

Osteoblasts
Chitosan
Microarrays
Oligonucleotide Array Sequence Analysis
Gene expression
Complementary DNA
Gene Expression
Polymerase Chain Reaction
Water
Phosphatases
Bioactivity
Alkaline Phosphatase
Cultured Cells
Cells
Tissue
Cell Line
Control Groups
oligochitosan

Keywords

  • cDNA microarray
  • Chitooligosaccharide
  • Gene expression
  • Osteoblast
  • RT-PCR

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide. / Ohara, Naoko; Hayashi, Yoshihiko; Yamada, Shizuka; Kim, Se Kwon; Matsunaga, Tsunenori; Yanagiguchi, Kajiro; Ikeda, Takeshi.

In: Biomaterials, Vol. 25, No. 10, 05.2004, p. 1749-1754.

Research output: Contribution to journalArticle

Ohara, Naoko ; Hayashi, Yoshihiko ; Yamada, Shizuka ; Kim, Se Kwon ; Matsunaga, Tsunenori ; Yanagiguchi, Kajiro ; Ikeda, Takeshi. / Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide. In: Biomaterials. 2004 ; Vol. 25, No. 10. pp. 1749-1754.
@article{f00636ffcb174f3c9e84181a0fe9eac6,
title = "Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide",
abstract = "Chitosan has a variety of biological activities. However, little is known about how chitosan modulates the hard tissue forming cells. When we cultured an osteoblastic cell line in α-MEM supplemented with 10{\%} FBS and 0.005{\%} chitooligosaccharide for 3 days, alkaline phosphatase (ALP) activity was significantly high compared with the control culture group (p",
keywords = "cDNA microarray, Chitooligosaccharide, Gene expression, Osteoblast, RT-PCR",
author = "Naoko Ohara and Yoshihiko Hayashi and Shizuka Yamada and Kim, {Se Kwon} and Tsunenori Matsunaga and Kajiro Yanagiguchi and Takeshi Ikeda",
year = "2004",
month = "5",
doi = "10.1016/j.biomaterials.2003.08.022",
language = "English",
volume = "25",
pages = "1749--1754",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "10",

}

TY - JOUR

T1 - Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide

AU - Ohara, Naoko

AU - Hayashi, Yoshihiko

AU - Yamada, Shizuka

AU - Kim, Se Kwon

AU - Matsunaga, Tsunenori

AU - Yanagiguchi, Kajiro

AU - Ikeda, Takeshi

PY - 2004/5

Y1 - 2004/5

N2 - Chitosan has a variety of biological activities. However, little is known about how chitosan modulates the hard tissue forming cells. When we cultured an osteoblastic cell line in α-MEM supplemented with 10% FBS and 0.005% chitooligosaccharide for 3 days, alkaline phosphatase (ALP) activity was significantly high compared with the control culture group (p

AB - Chitosan has a variety of biological activities. However, little is known about how chitosan modulates the hard tissue forming cells. When we cultured an osteoblastic cell line in α-MEM supplemented with 10% FBS and 0.005% chitooligosaccharide for 3 days, alkaline phosphatase (ALP) activity was significantly high compared with the control culture group (p

KW - cDNA microarray

KW - Chitooligosaccharide

KW - Gene expression

KW - Osteoblast

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=0345869861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345869861&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2003.08.022

DO - 10.1016/j.biomaterials.2003.08.022

M3 - Article

VL - 25

SP - 1749

EP - 1754

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 10

ER -