Early events responsible for aluminum toxicity symptoms in suspension-cultured tobacco cells

Mayandi Sivaguru, Yoko Yamamoto, Zdenko Rengel, Ju Ahn Sung, Hideaki Matsumoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We investigated the aluminum (Al)-induced alterations in zeta potential, plasma membrane (PM) potential and intracellular calcium levels to elucidate their interaction with callose production induced by Al toxicity. A noninvasive confocal laser microscopy has been used to analyse the live tobacco (Nicotiana tabacum) cell events by means of fluorescent probes Fluo-3 acetoxymethyl ester (intracellular calcium) and DiBAC4 (PM potential) as well as to monitor callose accumulation. Log-phase cells showed no detectable changes in the PM potential during the first 30 min of Al treatment, but sustained large depolarization from 60 min onwards. Measurement of zeta potential confirmed the depolarization effect of Al, but the kinetics were different. The Al-treated cells showed a moderate increase in intracellular Ca2+ levels and callose production in 1 h, which coincided with the time course of PM depolarization. Compared with the Al treatment, cyclopiazonic acid, an inhibitor of endoplasmic reticulum Ca2+-ATPase, facilitated a higher increase in intracellular Ca2+ levels, but resulted in accumulation of only moderate levels of callose. Calcium channel modulators and Al induced similar levels of callose in the initial 1 h of treatment. Callose production induced by Al toxicity is dependent on both depolarization of the PM and an increase in intracellular Ca2+ levels.

Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalNew Phytologist
Volume165
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Tobacco
Aluminum
Toxicity
aluminum
callose
Cultured Cells
Suspensions
tobacco
toxicity
Cell membranes
Depolarization
plasma membrane
Cell Membrane
calcium
membrane potential
cells
Membrane Potentials
Zeta potential
Confocal Microscopy
cyclopiazonic acid

Keywords

  • Al toxicity
  • Callose formation
  • Intracellular calcium
  • Nicotiana tabacum
  • Plasma membrane depolarization
  • Zeta potential

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Early events responsible for aluminum toxicity symptoms in suspension-cultured tobacco cells. / Sivaguru, Mayandi; Yamamoto, Yoko; Rengel, Zdenko; Sung, Ju Ahn; Matsumoto, Hideaki.

In: New Phytologist, Vol. 165, No. 1, 01.2005, p. 99-109.

Research output: Contribution to journalArticle

Sivaguru, Mayandi ; Yamamoto, Yoko ; Rengel, Zdenko ; Sung, Ju Ahn ; Matsumoto, Hideaki. / Early events responsible for aluminum toxicity symptoms in suspension-cultured tobacco cells. In: New Phytologist. 2005 ; Vol. 165, No. 1. pp. 99-109.
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