Early detection of elevated lactate levels in a mitochondrial disease model using chemical exchange saturation transfer (CEST) and magnetic resonance spectroscopy (MRS) at 7T-MRI

Shigeyoshi Saito, Yusuke Takahashi, Akiko Ohki, Yasunori Shintani, Takahiro Higuchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study aimed to use chemical exchange saturation transfer (CEST) and magnetic resonance spectroscopy (MRS) at 7T-MRI for early detection of intracerebral lactate in a mitochondrial disease model without brain lesions. We considered Ndufs4-knockout (KO) mice as Leigh syndrome models and wild-type (WT) mice as control mice. Brain MRI and 1 H-MRS were performed. T 2 WI data acquired with the Rapid Acquisition with Refocused Echoes (RARE) sequence were used for evaluation of brain lesions. CEST imaging of mice brains was performed using RARE with a magnetization transfer (MT) pulse. The MT ratio (MTR) asymmetry curves and five MTR asymmetry maps at 0.5, 1.0, 2.0, 3.0, and 3.5 ppm were calculated using these CEST images. Metabolite concentrations were measured by MRS. T 2 WI MRI revealed no obvious abnormal findings in KO and WT mice brains at 6 weeks of age. The MTR asymmetry maps at 0.5 ppm, 1.0 ppm, and 2.0 ppm of the KO mice were higher than those of the control mice. Brain 1 H MRS revealed a significant increase in lactate levels in all KO mice in comparison with those in the control mice. Additionally, creatine levels in the KO mice were slightly higher than those in the control mice. The levels of the other four metabolites—mIns, NAA + NAAG, GPC + PCh, and Glu + Gln—did not change significantly. We propose that CEST imaging can be used as a biomarker of intracerebral elevated lactate levels in mitochondrial disease.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalRadiological physics and technology
Volume12
Issue number1
DOIs
Publication statusPublished - Mar 20 2019
Externally publishedYes

Fingerprint

Chemical Models
Mitochondrial Diseases
lactates
magnetic resonance spectroscopy
mice
knockout mice
Lactic Acid
Knockout Mice
Magnetic Resonance Spectroscopy
brain
saturation
Brain
asymmetry
lesions
Leigh Disease
acquisition
echoes
creatine
Creatine
magnetization

Keywords

  • Chemical exchange saturation transfer
  • Magnetic resonance imaging
  • Magnetic resonance spectroscopy
  • Mitochondrial disease

ASJC Scopus subject areas

  • Radiation
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Radiology Nuclear Medicine and imaging

Cite this

Early detection of elevated lactate levels in a mitochondrial disease model using chemical exchange saturation transfer (CEST) and magnetic resonance spectroscopy (MRS) at 7T-MRI. / Saito, Shigeyoshi; Takahashi, Yusuke; Ohki, Akiko; Shintani, Yasunori; Higuchi, Takahiro.

In: Radiological physics and technology, Vol. 12, No. 1, 20.03.2019, p. 46-54.

Research output: Contribution to journalArticle

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