Early changes in basement membrane thickness in airway walls post-lung transplantation

Lucas Law, Ling Zheng, Bernadette Orsida, Bronwyn Levvey, Takahiro Oto, A. Thomas C. Kotsimbos, Gregory I. Snell, Trevor J. Williams

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Identification of early histopathologic markers of future bronchiolitis obliterans syndrome (BOS) may enable preemptive targeted intervention, delaying and perhaps preventing the onset of BOS. This study aimed to determine if early changes in airway epithelial basement membrane thickness predisposes transplant recipients to the subsequent development of BOS. Methods: Basement membrane thickness was measured in serial endobronchial biopsies taken from 29 initially stable lung transplant recipients (sLTR) recruited 148 ± 80 days post-transplant and followed for 3 years. A further 2 years of clinical follow-up was undertaken without biopsies to follow lung function and define ultimate BOS status. Nine healthy subjects (non-atopic, non-asthmatic) were recruited as controls. Sections of paraffinized endobronchial biopsies were stained for collagen type I immunohistochemically, and basement membrane thickness was assessed by computer image analysis. Results: BOS developed in 21 of 29 patients in the 5 years of follow-up, 16 of which had endobronchial biopsies available for analysis before BOS developed (ever-BOS). The first endobronchial biopsies showed increased BMT in the combined sLTR and ever-BOS patients compared with the controls. This initial increase in basement membrane thickness resolved to normal levels within 300 days post-transplant, with a strong negative correlation (r2 = 0.424, p < 0.0001) of basement membrane thickness vs time. Paradoxically, the sLTR tended to have the greatest basement membrane thickness at baseline. Conclusion: An initial increase in basement membrane thickness is seen in the airway walls of all lung transplant recipients. This is transient and does not appear to be a risk factor for the subsequent development of BOS in lung allograft recipients.

Original languageEnglish
Pages (from-to)1571-1576
Number of pages6
JournalJournal of Heart and Lung Transplantation
Volume24
Issue number10
DOIs
Publication statusPublished - Oct 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine
  • Transplantation

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    Law, L., Zheng, L., Orsida, B., Levvey, B., Oto, T., Kotsimbos, A. T. C., Snell, G. I., & Williams, T. J. (2005). Early changes in basement membrane thickness in airway walls post-lung transplantation. Journal of Heart and Lung Transplantation, 24(10), 1571-1576. https://doi.org/10.1016/j.healun.2005.01.007