E-selectin targeting to visualize tumors in vivo

Masahiko Hirai, Yoshie Hiramatsu, Shinki Iwashita, Takayuki Otani, Ling Chen, Yue Guang Li, Masashi Okada, Kazunori Oie, Koich Igarashi, Hideaki Wakita, Masaharu Seno

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10 Citations (Scopus)

Abstract

Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.5. The liposomes efficiently recognized human umbilical vein endothelial cells only when E-selectin was induced by angiogenic factors such as TNF-α in vitro. Cy5.5 encapsulated into liposomes that were conjugated with an anti-E-selectin antibody successfully visualized Ehrlich ascites tumor cells when transplanted into mice. Thus, E-selectin targeting with liposomes containing a near-infrared fluorescent dye was found effective in visualizing tumors in vivo. This strategy should be extremely useful as a method to identify sentinel lymphatic nodes and angiogenic tumors as well as use for drug delivery to tumor cells.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalContrast Media and Molecular Imaging
Volume5
Issue number2
DOIs
Publication statusPublished - Mar 1 2010

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Keywords

  • Drug delivery system
  • E-selectin
  • In vivo imaging
  • Liposome

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Hirai, M., Hiramatsu, Y., Iwashita, S., Otani, T., Chen, L., Li, Y. G., Okada, M., Oie, K., Igarashi, K., Wakita, H., & Seno, M. (2010). E-selectin targeting to visualize tumors in vivo. Contrast Media and Molecular Imaging, 5(2), 70-77. https://doi.org/10.1002/cmmi.367