Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells

Atsushi Otsuka, Tadashi Abe, Masami Watanabe, Hitoshi Yagisawa, Kohji Takei, Hiroshi Yamada

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Dynamin 2 has been reported to be implicated in phagocytosis. However, the mode of action of dynamin is poorly understood. In this study, we examined whether dynamin 2 participates in actin assembly during phagocytosis in Sertoli cells. In the presence of dynasore, a dynamin inhibitor, phagocytosis was reduced by 60-70% in Sertoli cells and macrophages. Scanning electron microscopy revealed that Sertoli cells treated with dynasore were unable to form phagocytic cups. In addition, dysfunction of dynamin 2 reduced both actin polymerization and recruitment of actin and dynamin 2 to phosphatidylinositol (4,5) bisphosphate [PI(4,5)P2]-containing liposomes. The formation of dynamin 2-positive ruffles of Sertoli cells was decreased by 60-70% by sequestering PI(4,5)P2 either by expression of PH domain of PLCδ or treatment with neomycin. These results strongly suggest that dynamin 2 is involved in actin dynamics and the formation of dynamin 2-positive ruffles during phagocytosis.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number3
DOIs
Publication statusPublished - Jan 16 2009

Fingerprint

Dynamin II
Sertoli Cells
Phagocytosis
Actins
Dynamins
Neomycin
Macrophages
Programmable logic controllers
Phosphatidylinositols
Liposomes
Polymerization
Electron Scanning Microscopy
Scanning electron microscopy

Keywords

  • Actin dynamics
  • Dynamin
  • Endocytosis
  • Phagocytosis
  • Testis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells. / Otsuka, Atsushi; Abe, Tadashi; Watanabe, Masami; Yagisawa, Hitoshi; Takei, Kohji; Yamada, Hiroshi.

In: Biochemical and Biophysical Research Communications, Vol. 378, No. 3, 16.01.2009, p. 478-482.

Research output: Contribution to journalArticle

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