Dynamin 2 cooperates with amphiphysin 1 in phagocytosis in Sertoli cells

Akira Nakanishi, Tadashi Abe, Masami Watanabe, Kohji Takei, Hiroshi Yamada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound in vitro. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested. Copyright

Original languageEnglish
Article number5
JournalActa medica Okayama
Volume62
Issue number6
Publication statusPublished - Dec 1 2008

    Fingerprint

Keywords

  • Amphiphysin
  • Dynamin
  • Phagocytosis
  • Testis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this