Abstract
Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.
| Original language | English |
|---|---|
| Article number | Article 12 |
| Journal | Biology of Reproduction |
| Volume | 90 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Crustacean hyperglycemic hormone
- Eyestalk ablation
- Hormone
- Invertebrates
- Litopenaeus vannamei
- Mechanisms of hormone action
- Molt stage
- Neuropeptides
- Time-resolved fluoroimmunoassay
- Vitellogenesis-Inhibiting hormone
- Vitellogenin
ASJC Scopus subject areas
- Cell Biology
Cite this
Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei : Relation to molting and eyestalk ablation. / Kang, Bong Jung; Okutsu, Tomoyuki; Tsutsui, Naoaki; Shinji, Junpei; Bae, Sun Hye; Wilder, Marcy N.
In: Biology of Reproduction, Vol. 90, No. 1, Article 12, 2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei
T2 - Relation to molting and eyestalk ablation
AU - Kang, Bong Jung
AU - Okutsu, Tomoyuki
AU - Tsutsui, Naoaki
AU - Shinji, Junpei
AU - Bae, Sun Hye
AU - Wilder, Marcy N.
PY - 2014
Y1 - 2014
N2 - Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.
AB - Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.
KW - Crustacean hyperglycemic hormone
KW - Eyestalk ablation
KW - Hormone
KW - Invertebrates
KW - Litopenaeus vannamei
KW - Mechanisms of hormone action
KW - Molt stage
KW - Neuropeptides
KW - Time-resolved fluoroimmunoassay
KW - Vitellogenesis-Inhibiting hormone
KW - Vitellogenin
UR - http://www.scopus.com/inward/record.url?scp=84899943518&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899943518&partnerID=8YFLogxK
U2 - 10.1095/biolreprod.113.112243
DO - 10.1095/biolreprod.113.112243
M3 - Article
VL - 90
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 1
M1 - Article 12
ER -