Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei

Relation to molting and eyestalk ablation

Bong Jung Kang, Tomoyuki Okutsu, Naoaki Tsutsui, Junpei Shinji, Sun Hye Bae, Marcy N. Wilder

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.

Original languageEnglish
Article numberArticle 12
JournalBiology of Reproduction
Volume90
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Vitellogenins
Molting
Hemolymph
Fluoroimmunoassay
vitellogenesis inhibiting hormone
Menstrual Cycle
Messenger RNA
Peptides
Antibodies

Keywords

  • Crustacean hyperglycemic hormone
  • Eyestalk ablation
  • Hormone
  • Invertebrates
  • Litopenaeus vannamei
  • Mechanisms of hormone action
  • Molt stage
  • Neuropeptides
  • Time-resolved fluoroimmunoassay
  • Vitellogenesis-Inhibiting hormone
  • Vitellogenin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei : Relation to molting and eyestalk ablation. / Kang, Bong Jung; Okutsu, Tomoyuki; Tsutsui, Naoaki; Shinji, Junpei; Bae, Sun Hye; Wilder, Marcy N.

In: Biology of Reproduction, Vol. 90, No. 1, Article 12, 2014.

Research output: Contribution to journalArticle

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abstract = "Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.",
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AU - Okutsu, Tomoyuki

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AU - Shinji, Junpei

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AU - Wilder, Marcy N.

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