Dynamics of tandem repetitive Afa-family sequences in Triticeae, wheat- related species

Kiyotaka Nagaki, Hisashi Tsujimoto, Tetsuo Sasakuma

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Afa-family sequences in wheat-related species, Triticeae, are tandem repetitive sequences of 340 bp. All the analyzed Triticeae species carried the sequences in their genomes, though the copy numbers varied about 100- fold among the species. The nucleotide fragments amplified by PCR were cloned and sequenced, and their behavior in the evolution of Triticeae was analyzed by the neighbor-joining (NJ) method. The sequences in genomes with many copies of this family clustered at independent branches of the phylogenic tree, whereas the sequences in genomes with a few copies did not. This may suggest that Afa-family sequences had amplified several times in the evolution of Triticeae, each using a limited number of different master copies. In addition, the sequences of the A and B genomes of hexaploid common wheat indicated that the Afa-family sequences had not evolved in a concerted manner between the genomes. Furthermore, the sequences of each chromosome of the D genome of this species indicated that the sequences had amplified on all over the D-genome chromosomes in a short period.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalJournal of Molecular Evolution
Volume47
Issue number2
DOIs
Publication statusPublished - Aug 24 1998
Externally publishedYes

Keywords

  • Afa family
  • Tandem repeat amplification
  • Triticeae
  • Wheat

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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