Dynamic interaction of amphiphysin with N-WASP regulates actin assembly

Hiroshi Yamada, Sergi Padilla-Parra, Sun Joo Park, Toshiki Itoh, Mathilde Chaineau, Ilaria Monaldi, Ottavio Cremona, Fabio Benfenati, Pietro De Camilli, Maïté Coppey-Moisan, Marc Tramier, Thierry Galli, Kohji Takei

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependentfission, wasalsoshowntohavearegulatoryroleinactindynamics. Here, we report that amphiphysin 1 interacts with N-WASP and stimulates N-WASP- and Arp2/ 3-dependent actin polymerization. Both the Src homology 3 and the N-BAR domains are required for this stimulation. Acidic liposome-triggered, N-WASP-dependent actin polymerization is strongly impaired in brain cytosol of amphiphysin 1 knock-out mice. FRET-FLIM analysis of Sertoli cells, where endogenously expressed amphiphysin 1 colocalizes with N-WASP in peripheral ruffles, confirmed the association between the two proteins in vivo. This association undergoes regulation and is enhanced by stimulating phosphatidylserine receptors on the cell surface with phosphatidylserine- containing liposomes that trigger ruffle formation. These results indicate that actin regulation is a key function of amphiphysin 1 and that such function cooperates with the endocytic adaptor role and membrane shaping/curvature sensing properties of the protein during the endocytic reaction.

Original languageEnglish
Pages (from-to)34244-34256
Number of pages13
JournalJournal of Biological Chemistry
Volume284
Issue number49
DOIs
Publication statusPublished - Dec 4 2009

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Actins
Liposomes
Polymerization
Association reactions
Dynamins
Clathrin
Sertoli Cells
Phosphatidylserines
Endocytosis
Knockout Mice
Cytosol
Synapses
Brain
Proteins
amphiphysin
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. / Yamada, Hiroshi; Padilla-Parra, Sergi; Park, Sun Joo; Itoh, Toshiki; Chaineau, Mathilde; Monaldi, Ilaria; Cremona, Ottavio; Benfenati, Fabio; De Camilli, Pietro; Coppey-Moisan, Maïté; Tramier, Marc; Galli, Thierry; Takei, Kohji.

In: Journal of Biological Chemistry, Vol. 284, No. 49, 04.12.2009, p. 34244-34256.

Research output: Contribution to journalArticle

Yamada, H, Padilla-Parra, S, Park, SJ, Itoh, T, Chaineau, M, Monaldi, I, Cremona, O, Benfenati, F, De Camilli, P, Coppey-Moisan, M, Tramier, M, Galli, T & Takei, K 2009, 'Dynamic interaction of amphiphysin with N-WASP regulates actin assembly', Journal of Biological Chemistry, vol. 284, no. 49, pp. 34244-34256. https://doi.org/10.1074/jbc.M109.064204
Yamada H, Padilla-Parra S, Park SJ, Itoh T, Chaineau M, Monaldi I et al. Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. Journal of Biological Chemistry. 2009 Dec 4;284(49):34244-34256. https://doi.org/10.1074/jbc.M109.064204
Yamada, Hiroshi ; Padilla-Parra, Sergi ; Park, Sun Joo ; Itoh, Toshiki ; Chaineau, Mathilde ; Monaldi, Ilaria ; Cremona, Ottavio ; Benfenati, Fabio ; De Camilli, Pietro ; Coppey-Moisan, Maïté ; Tramier, Marc ; Galli, Thierry ; Takei, Kohji. / Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 49. pp. 34244-34256.
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