Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken

Shigetoshi Yokoyama, Megumi Hashimoto, Hirohito Shimizu, Hiroe Ueno-Kudoh, Kenta Uchibe, Ichiro Kimura, Hiroshi Asahara

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The Caenorhabditis elegans heterochronic gene lin-28 regulates developmental timing in the nematode trunk. We report the dynamic expression patterns of Lin-28 homologues in mouse and chick embryos. Whole mount in situ hybridization revealed specific and intriguing expression patterns of Lin-28 in the developing mouse and chick limb bud. Mouse Lin-28 expression was detected in both the forelimb and hindlimb at E9.5, but disappeared from the forelimb at E10.5, and finally from the forelimb and hindlimb at E11.5. Chicken Lin-28, which was first detected in the limb primordium at stage 15/16, was also downregulated as the stage proceeded. The amino acid sequences of mouse and chicken Lin-28 genes are highly conserved and the similar expression patterns of Lin-28 during limb development in mouse and chicken suggest that this heterochronic gene is also conserved during vertebrate limb development.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalGene Expression Patterns
Volume8
Issue number3
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Embryonic Development
Chickens
Forelimb
Gene Expression
Extremities
Hindlimb
Genes
Limb Buds
Caenorhabditis elegans
Chick Embryo
In Situ Hybridization
Vertebrates
Amino Acid Sequence
Down-Regulation

Keywords

  • Chick
  • Developmental timing
  • Dynamic expression
  • Embryo
  • Heterochronic gene
  • Limb bud
  • Lin-28
  • MicroRNAs
  • Mouse
  • Whole mount in situ hybridization

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Yokoyama, S., Hashimoto, M., Shimizu, H., Ueno-Kudoh, H., Uchibe, K., Kimura, I., & Asahara, H. (2008). Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken. Gene Expression Patterns, 8(3), 155-160. https://doi.org/10.1016/j.gep.2007.11.001

Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken. / Yokoyama, Shigetoshi; Hashimoto, Megumi; Shimizu, Hirohito; Ueno-Kudoh, Hiroe; Uchibe, Kenta; Kimura, Ichiro; Asahara, Hiroshi.

In: Gene Expression Patterns, Vol. 8, No. 3, 02.2008, p. 155-160.

Research output: Contribution to journalArticle

Yokoyama, S, Hashimoto, M, Shimizu, H, Ueno-Kudoh, H, Uchibe, K, Kimura, I & Asahara, H 2008, 'Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken', Gene Expression Patterns, vol. 8, no. 3, pp. 155-160. https://doi.org/10.1016/j.gep.2007.11.001
Yokoyama, Shigetoshi ; Hashimoto, Megumi ; Shimizu, Hirohito ; Ueno-Kudoh, Hiroe ; Uchibe, Kenta ; Kimura, Ichiro ; Asahara, Hiroshi. / Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken. In: Gene Expression Patterns. 2008 ; Vol. 8, No. 3. pp. 155-160.
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