Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus

Taro Mito, Taro Nakamura, Isao Sarashina, Chun Che Chang, Shotaro Ogawa, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The specification of germ cells during embryogenesis is an important issue in the development of metazoans. In insects, the mode of germ cell specification appears to be highly variable among species and molecular data are not sufficient to provide an evolutionary perspective to this issue. Expression of vasa can be used as a germ line marker. Here, we report the isolation of a vasa-like gene in a hemimetabolous insect, the cricket Gryllus bimaculatus (Gb'vas), and its expression patterns during oogenesis and embryogenesis. Gb'vas is preferentially expressed in the germarium and the expression of Gb'vas is detectable throughout vitellogenesis including mature eggs subjected to oviposition, suggesting that Gb'vas is maternally contributed to the cricket eggs. The zygotic expression of Gb'vas appears to start at the mid blastoderm stage in the posterior region of the egg, expanding in a developing germ anlage. In early germbands, an intense expression of Gb'vas is restricted to the posterior end. In later embryos, Gb'vas expression extends over the whole body and then distinctly localized to the embryonic gonad at the stage immediately before hatching. These results suggest that, in the cricket, germ cells are specified early in development at the posterior end of an early germband, as proposed by Heymons (1895) based on cytological criteria.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalDevelopment Genes and Evolution
Volume218
Issue number7
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

Fingerprint

Gryllidae
Gryllus bimaculatus
germ cell
cricket
Germ Cells
Embryonic Development
germ cells
embryogenesis
egg
insect
Eggs
Insects
Blastoderm
Vitellogenesis
blastoderm
oviposition
Oogenesis
Oviposition
insects
hatching

Keywords

  • Gryllus bimaculatus
  • Hemimetabolous insect
  • Primordial germ cells
  • Vasa

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus. / Mito, Taro; Nakamura, Taro; Sarashina, Isao; Chang, Chun Che; Ogawa, Shotaro; Ohuchi, Hideyo; Noji, Sumihare.

In: Development Genes and Evolution, Vol. 218, No. 7, 07.2008, p. 381-387.

Research output: Contribution to journalArticle

Mito, Taro ; Nakamura, Taro ; Sarashina, Isao ; Chang, Chun Che ; Ogawa, Shotaro ; Ohuchi, Hideyo ; Noji, Sumihare. / Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus. In: Development Genes and Evolution. 2008 ; Vol. 218, No. 7. pp. 381-387.
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