@article{891a41040f4240bc9c2f834692ca7da9,
title = "Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis",
abstract = "Dynamin is a mechanochemical GTPase essential for membrane fission during clathrin-mediated endocytosis. Dynamin forms helical complexes at the neck of clathrin-coated pits and their structural changes coupled with GTP hydrolysis drive membrane fission. Dynamin and its binding protein amphiphysin cooperatively regulate membrane remodeling during the fission, but its precise mechanism remains elusive. In this study, we analyzed structural changes of dynamin-amphiphysin complexes during the membrane fission using electron microscopy (EM) and high-speed atomic force microscopy (HS-AFM). Interestingly, HS-AFM analyses show that the dynamin-amphiphysin helices are rearranged to form clusters upon GTP hydrolysis and membrane constriction occurs at protein-uncoated regions flanking the clusters. We also show a novel function of amphiphysin in size control of the clusters to enhance biogenesis of endocytic vesicles. Our approaches using combination of EM and HS-AFM clearly demonstrate new mechanistic insights into the dynamics of dynamin-amphiphysin complexes during membrane fission.",
author = "Tetsuya Takeda and Toshiya Kozai and Huiran Yang and Daiki Ishikuro and Kaho Seyama and Yusuke Kumagai and Tadashi Abe and Hiroshi Yamada and Takayuki Uchihashi and Toshio Ando and Kohji Takei",
note = "Funding Information: The authors thank Dr. Harvey McMahon (MRC-LMB) for sharing valuable reagent (NFA Galactocerebrosides). The authors also thank Dr. Aur{\'e}lien Roux and Dr. Adai Colom (University of Geneva), Dr. Lorena Redondo-Morata (INSERM U1006/Aix-Marseille Universit{\'e}) and Dr. Masatoshi Ichikawa (Kyoto University) for their critical reading of the manuscript. This work was supported by JST/CREST program (#JPMJCR13M1) (to TA and KT). This work was also supported in part by JSPS KAKENHI Grant Numbers JP15H03540 (to TU), MEXT KAKENHI Grant Numbers JP16H00830 and JP16H00758 (to TU). Funding Information: The authors thank Dr. Harvey McMahon (MRC-LMB) for sharing valuable reagent (NFA Galactocerebrosides). The authors also thank Dr. Aur{\'e} lien Roux and Dr. Adai Colom (University of Geneva), Dr. Lorena Redondo-Morata (INSERM U1006/Aix-Marseille Universite´) and Dr. Masatoshi Ichikawa (Kyoto University) for their critical reading of the manuscript. This work was supported byJST/CREST program (#JPMJCR13M1) (to TA and KT). This work was also supported in part by JSPS KAKENHI Grant Numbers JP15H03540 (to TU), MEXT KAKENHI Grant Numbers JP16H00830 and JP16H00758 (to TU). Publisher Copyright: {\textcopyright} Takeda et al.",
year = "2018",
month = jan,
day = "23",
doi = "10.7554/eLife.30246",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}