Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation

Masatoki Taga, Dai Tsuchiya, Minoru Murata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.

Original languageEnglish
Pages (from-to)1012-1020
Number of pages9
JournalMycological Research
Volume107
Issue number9
DOIs
Publication statusPublished - Sep 1 2003

Fingerprint

Ribosomal DNA
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Mitosis
mitosis
condensation
Fungi
fluorescence
fungus
metaphase
fungi
Fusarium eumartii
Ascomycota
anaphase
prophase
Metaphase
interphase
chromosomes
chromosome
Prophase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation. / Taga, Masatoki; Tsuchiya, Dai; Murata, Minoru.

In: Mycological Research, Vol. 107, No. 9, 01.09.2003, p. 1012-1020.

Research output: Contribution to journalArticle

Taga, Masatoki ; Tsuchiya, Dai ; Murata, Minoru. / Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation. In: Mycological Research. 2003 ; Vol. 107, No. 9. pp. 1012-1020.
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