Dynamic changes of mitochondrial fusion and fission proteins after transient cerebral ischemia in mice

Wentao Liu, Fengfeng Tian, Tomoko Kurata, Nobutoshi Morimoto, Koji Abe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

With fusion or fission, mitochondria alter their morphology in response to various physiological and pathological stimuli, resulting in elongated, tubular, interconnected, or fragmented forms. Immunohistochemistry and Western blot analysis were performed at 2 days, 7 days, 14 days, and 28 days after 90 min of transient middle cerebral artery occlusion (tMCAO) in mice. This study showed that mitochondrial fission protein dynamin-related protein 1 (Drp1) and fusion protein optic atrophy 1 (Opa1) were both upregulated in the ischemic penumbra, with the peak at 2 days after tMCAO, whereas phosphorylated-Drp1 (P-Drp1) progressively increased with a peak at 14 days after tMCAO. Double-immunofluorescence analysis showed many Drp1/cytochrome c oxidase subunit l (COX1) double-positive cells and Opa1/COX1 double-positive cells in the ischemic penumbra and also showed some double-positive cells with Drp1/terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) and Opa1/TUNEL in the ischemic penumbra. In contrast, both Drp1 and Opa1 showed progressive decreases until 2 days after tMCAO in the ischemic core because of necrotic brain damage. The present study suggests that there was a continuous mitochondrial fission and fusion during these periods in the ischemic penumbra after tMCAO, probably in an effort toward mitophagy and cellular survival.

Original languageEnglish
Pages (from-to)1183-1189
Number of pages7
JournalJournal of Neuroscience Research
Volume90
Issue number6
DOIs
Publication statusPublished - Jun 2012

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Mitochondrial Dynamics
Dynamins
Transient Ischemic Attack
Autosomal Dominant Optic Atrophy
Middle Cerebral Artery Infarction
Digoxigenin
Proteins
Mitochondrial Degradation
DNA Nucleotidylexotransferase
Mitochondrial Proteins
Electron Transport Complex IV
Transferases
Fluorescent Antibody Technique
Mitochondria
Western Blotting
Immunohistochemistry
Brain

Keywords

  • Drp1
  • Fission
  • Fusion
  • Opa1
  • TMCAO

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Dynamic changes of mitochondrial fusion and fission proteins after transient cerebral ischemia in mice. / Liu, Wentao; Tian, Fengfeng; Kurata, Tomoko; Morimoto, Nobutoshi; Abe, Koji.

In: Journal of Neuroscience Research, Vol. 90, No. 6, 06.2012, p. 1183-1189.

Research output: Contribution to journalArticle

Liu, Wentao ; Tian, Fengfeng ; Kurata, Tomoko ; Morimoto, Nobutoshi ; Abe, Koji. / Dynamic changes of mitochondrial fusion and fission proteins after transient cerebral ischemia in mice. In: Journal of Neuroscience Research. 2012 ; Vol. 90, No. 6. pp. 1183-1189.
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