Abstract
With fusion or fission, mitochondria alter their morphology in response to various physiological and pathological stimuli resulting in either elongated, tubular, interconnected or fragmented form. Immunohistochemistry and Western blot analyses were performed at 2, 7, 14 and 28 d after 90 min of transient middle cerebral artery occlusion (tMCAO) in mice. The present study showed that mitochondrial fission protein fission 1 (Fis1) and phosphorylated dynamin-related protein 1 (P-Drp1) both progressively increased with the peak at 14 d after tMCAO. Double immunofluorescent analysis showed the number of double positive cells with Fis1/Drp1 reduced between 2 and 28 d after 90 min of tMCAO, and also showed some double positive cells with Fis1/terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) in the peri-infract regions at 2 d after the reperfusion. The present study suggests a progressive activation of mitochondrial fission proteins Fis1 and P-Drp1 in relation to apoptotic process in neural cells of the peri-infract regions after tMCAO.
| Original language | English |
|---|---|
| Pages (from-to) | 94-99 |
| Number of pages | 6 |
| Journal | Brain Research |
| Volume | 1456 |
| DOIs | |
| Publication status | Published - May 25 2012 |
Fingerprint
Keywords
- Fis1
- Fission
- Mitochondria
- Peri-infract regions
- tMCAO
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Dynamic changes of mitochondrial fission proteins after transient cerebral ischemia in mice. / Liu, Wentao; Tian, Fengfeng; Kurata, Tomoko; Morimoto, Nobutoshi; Abe, Koji.
In: Brain Research, Vol. 1456, 25.05.2012, p. 94-99.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic changes of mitochondrial fission proteins after transient cerebral ischemia in mice
AU - Liu, Wentao
AU - Tian, Fengfeng
AU - Kurata, Tomoko
AU - Morimoto, Nobutoshi
AU - Abe, Koji
PY - 2012/5/25
Y1 - 2012/5/25
N2 - With fusion or fission, mitochondria alter their morphology in response to various physiological and pathological stimuli resulting in either elongated, tubular, interconnected or fragmented form. Immunohistochemistry and Western blot analyses were performed at 2, 7, 14 and 28 d after 90 min of transient middle cerebral artery occlusion (tMCAO) in mice. The present study showed that mitochondrial fission protein fission 1 (Fis1) and phosphorylated dynamin-related protein 1 (P-Drp1) both progressively increased with the peak at 14 d after tMCAO. Double immunofluorescent analysis showed the number of double positive cells with Fis1/Drp1 reduced between 2 and 28 d after 90 min of tMCAO, and also showed some double positive cells with Fis1/terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) in the peri-infract regions at 2 d after the reperfusion. The present study suggests a progressive activation of mitochondrial fission proteins Fis1 and P-Drp1 in relation to apoptotic process in neural cells of the peri-infract regions after tMCAO.
AB - With fusion or fission, mitochondria alter their morphology in response to various physiological and pathological stimuli resulting in either elongated, tubular, interconnected or fragmented form. Immunohistochemistry and Western blot analyses were performed at 2, 7, 14 and 28 d after 90 min of transient middle cerebral artery occlusion (tMCAO) in mice. The present study showed that mitochondrial fission protein fission 1 (Fis1) and phosphorylated dynamin-related protein 1 (P-Drp1) both progressively increased with the peak at 14 d after tMCAO. Double immunofluorescent analysis showed the number of double positive cells with Fis1/Drp1 reduced between 2 and 28 d after 90 min of tMCAO, and also showed some double positive cells with Fis1/terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) in the peri-infract regions at 2 d after the reperfusion. The present study suggests a progressive activation of mitochondrial fission proteins Fis1 and P-Drp1 in relation to apoptotic process in neural cells of the peri-infract regions after tMCAO.
KW - Fis1
KW - Fission
KW - Mitochondria
KW - Peri-infract regions
KW - tMCAO
UR - http://www.scopus.com/inward/record.url?scp=84860640762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860640762&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2012.03.038
DO - 10.1016/j.brainres.2012.03.038
M3 - Article
C2 - 22498177
AN - SCOPUS:84860640762
VL - 1456
SP - 94
EP - 99
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -