Dynamic changes of cortical NADH fluorescence in rat focal ischemia: Normothermia vs. hypothermia

Toshihiro Sasaki, Yoshimasa Takeda, Minako Arai, Hideki Taninishi, Motomu Kobayashi, Kiyoshi Morita

Research output: Contribution to journalArticle

Abstract

[INTRODUCTION] Preischemic hypothermia has been suggested to reduce infarct volume. However, the dynamics of the growth of infarct volume during hypothermia has not been observed. In this study, we examined differences in expansion of the ischemic core in normothemic and hypothermic groups using a cortical NADH fluorescence imaging technique described by Higuchi et al. (1) [METHODS] Nine male spontaneous hypertensive rats weighing 300±20 g were used in this study. Permanent occlusion of the left common carotid and middle cerebral arteries was performed. In the hypothermic group (n=4), hypothermia was induced before ischemia, and then the animals were kept at 30-31°C for two hours and rewarmed to 36.5°C over a period of 60 minutes. In the normothermic group (n=5), the animals were kept at 37.0±0.5°C. A large cranial window was made on the left parietal-temporal bone, and cortical NADH fluorescence in this window was examined. The infarct volumes were measured 24 hours after onset of ischemia. [RESULTS] The areas of the ischemic core in the normothermic and hypothermic groups had increased to a maximal level at about 30 minutes and 60 minutes respectively. The areas of the ischemic core at 15, 30, 60 and 120 minutes after onset of ischemia and at the end of rewarming were significantly reduced in the hypothermic groups compared to those in the normothermic group.(see figure) The infarct volumes were also significantly reduced in the hypothermic group (P

Original languageEnglish
JournalJournal of Cerebral Blood Flow and Metabolism
Volume27
Issue numberSUPPL. 1
Publication statusPublished - Nov 13 2007

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Hypothermia
NAD
Ischemia
Fluorescence
Parietal Bone
Induced Hypothermia
Rewarming
Temporal Bone
Optical Imaging
Middle Cerebral Artery
Growth

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

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Dynamic changes of cortical NADH fluorescence in rat focal ischemia : Normothermia vs. hypothermia. / Sasaki, Toshihiro; Takeda, Yoshimasa; Arai, Minako; Taninishi, Hideki; Kobayashi, Motomu; Morita, Kiyoshi.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 27, No. SUPPL. 1, 13.11.2007.

Research output: Contribution to journalArticle

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AU - Sasaki, Toshihiro

AU - Takeda, Yoshimasa

AU - Arai, Minako

AU - Taninishi, Hideki

AU - Kobayashi, Motomu

AU - Morita, Kiyoshi

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