Abstract
Defensins are cationic antimicrobial peptides with a broad spectrum. Recently human β-defensin 2 (hBD-2) has been isolated from psoriatic skin; however, its exact localization and fate have not been fully understood. We studied the distribution pattern of hBD-2 in skin tissues of psoriasis and other inflammatory skin diseases. In the upper spinous and granular layer of pso-riasis vulgaris hBD-2 was present in the cytoplasm. In the horny layer the positive signals were in a basket-weave pattern, indicating possible accumulation of hBD-2 in the intercellular space. The similar pattern of hBD-2 distribution was observed in the lesions of num-mular eczema and atopic dermatitis. hBD-2 was not detected in the section of normal elbow and knee skin. When isolated psoriatic scales were stained, hBD-2 was detected in a wrapping paper-like distribution pattern surrounding the corneocytes. In horny layer of psoriatic skin hBD-2 was closely associated or colocalized with elafin, which is known to be in extracellular space, as demonstrated by double staining. Western blot analysis using cultured human keratinocytes detected hBD-2 with an expected size in the conditioned medium and in the cell lysates when stimulated with 5% FCS or IL-a. These results indicate that hBD-2 was synthesized and remained in cytoplasm in the upper spinous and granular layer, and then secreted into intercellular space in the horny layer. This dynamic change in hBD-2 distribution in epidermis is certainly relevant to function as an innate host defense mechanism against invading micro-organisms.
| Original language | English |
|---|---|
| Pages (from-to) | 678-684 |
| Number of pages | 7 |
| Journal | Journal of Molecular Medicine |
| Volume | 80 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Antimicrobial peptide
- Horny layer
- Human β-defensin
- Innate immunity
- Psoriasis
ASJC Scopus subject areas
- Medicine(all)
Cite this
Dynamic alteration of human β-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin. / Huh, Wook Kang; Oono, Takashi; Shirafuji, Yoshinori; Akiyama, Hisanori; Arata, Jirô; Sakaguchi, Masakiyo; Huh, Nam ho; Iwatsuki, Keiji.
In: Journal of Molecular Medicine, Vol. 80, No. 10, 2002, p. 678-684.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic alteration of human β-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin
AU - Huh, Wook Kang
AU - Oono, Takashi
AU - Shirafuji, Yoshinori
AU - Akiyama, Hisanori
AU - Arata, Jirô
AU - Sakaguchi, Masakiyo
AU - Huh, Nam ho
AU - Iwatsuki, Keiji
PY - 2002
Y1 - 2002
N2 - Defensins are cationic antimicrobial peptides with a broad spectrum. Recently human β-defensin 2 (hBD-2) has been isolated from psoriatic skin; however, its exact localization and fate have not been fully understood. We studied the distribution pattern of hBD-2 in skin tissues of psoriasis and other inflammatory skin diseases. In the upper spinous and granular layer of pso-riasis vulgaris hBD-2 was present in the cytoplasm. In the horny layer the positive signals were in a basket-weave pattern, indicating possible accumulation of hBD-2 in the intercellular space. The similar pattern of hBD-2 distribution was observed in the lesions of num-mular eczema and atopic dermatitis. hBD-2 was not detected in the section of normal elbow and knee skin. When isolated psoriatic scales were stained, hBD-2 was detected in a wrapping paper-like distribution pattern surrounding the corneocytes. In horny layer of psoriatic skin hBD-2 was closely associated or colocalized with elafin, which is known to be in extracellular space, as demonstrated by double staining. Western blot analysis using cultured human keratinocytes detected hBD-2 with an expected size in the conditioned medium and in the cell lysates when stimulated with 5% FCS or IL-a. These results indicate that hBD-2 was synthesized and remained in cytoplasm in the upper spinous and granular layer, and then secreted into intercellular space in the horny layer. This dynamic change in hBD-2 distribution in epidermis is certainly relevant to function as an innate host defense mechanism against invading micro-organisms.
AB - Defensins are cationic antimicrobial peptides with a broad spectrum. Recently human β-defensin 2 (hBD-2) has been isolated from psoriatic skin; however, its exact localization and fate have not been fully understood. We studied the distribution pattern of hBD-2 in skin tissues of psoriasis and other inflammatory skin diseases. In the upper spinous and granular layer of pso-riasis vulgaris hBD-2 was present in the cytoplasm. In the horny layer the positive signals were in a basket-weave pattern, indicating possible accumulation of hBD-2 in the intercellular space. The similar pattern of hBD-2 distribution was observed in the lesions of num-mular eczema and atopic dermatitis. hBD-2 was not detected in the section of normal elbow and knee skin. When isolated psoriatic scales were stained, hBD-2 was detected in a wrapping paper-like distribution pattern surrounding the corneocytes. In horny layer of psoriatic skin hBD-2 was closely associated or colocalized with elafin, which is known to be in extracellular space, as demonstrated by double staining. Western blot analysis using cultured human keratinocytes detected hBD-2 with an expected size in the conditioned medium and in the cell lysates when stimulated with 5% FCS or IL-a. These results indicate that hBD-2 was synthesized and remained in cytoplasm in the upper spinous and granular layer, and then secreted into intercellular space in the horny layer. This dynamic change in hBD-2 distribution in epidermis is certainly relevant to function as an innate host defense mechanism against invading micro-organisms.
KW - Antimicrobial peptide
KW - Horny layer
KW - Human β-defensin
KW - Innate immunity
KW - Psoriasis
UR - http://www.scopus.com/inward/record.url?scp=0036460781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036460781&partnerID=8YFLogxK
U2 - 10.1007/s00109-002-0373-z
DO - 10.1007/s00109-002-0373-z
M3 - Article
VL - 80
SP - 678
EP - 684
JO - Journal of Molecular Medicine
JF - Journal of Molecular Medicine
SN - 0946-2716
IS - 10
ER -