Durability of oxygen evolution of photosystem II incorporated into lipid bilayers

Tomoyasu Noji, Masaharu Kondo, Keisuke Kawakami, Jian Ren Shen, Mamoru Nango, Takehisa Dewa

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Photosystem II (PSII) has attracted a lot of attention for use in the construction of artificial photosynthetic materials due to its high activity of oxidation of water molecules. However, the robustness of PSII needs to be improved for in vitro application. In this study, we incorporated PSII (Thermosynechococcus vulcanus) into various phospholipid membranes to examine the activity and durability of oxygen evolution. PSII was incorporated into anionic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (PSII-DOPG), zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (PSII-DOPC), and cationic 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (PSII-EDOPC). Structural integrity of PSII was examined by absorption and fluorescence spectroscopy. Compared with PSII dissolved in a micellar solution of n-dodecyl-β-d-maltoside (PSII-micelle), durability of PSII-DOPC and PSII-DOPG were enhanced by 1.3- and 1.5-fold, respectively. The activity and durability of PSII-EDOPC was significantly low. Lipid-dependent activity and durability were discussed in terms of kinetic parameters of V max and K m, and inhibition of the electron acceptor, phenyl-p-benzoquinone.

    Original languageEnglish
    Pages (from-to)3231-3241
    Number of pages11
    JournalResearch on Chemical Intermediates
    Volume40
    Issue number9
    DOIs
    Publication statusPublished - Nov 2014

    Keywords

    • Lipids bilayers
    • Oxygen evolution activity
    • Phosphatidylglycerol
    • Photosynthesis
    • Photosystem II
    • Proteoliposome

    ASJC Scopus subject areas

    • Chemistry(all)

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