Abstract
Photosystem II (PSII) has attracted a lot of attention for use in the construction of artificial photosynthetic materials due to its high activity of oxidation of water molecules. However, the robustness of PSII needs to be improved for in vitro application. In this study, we incorporated PSII (Thermosynechococcus vulcanus) into various phospholipid membranes to examine the activity and durability of oxygen evolution. PSII was incorporated into anionic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (PSII-DOPG), zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (PSII-DOPC), and cationic 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (PSII-EDOPC). Structural integrity of PSII was examined by absorption and fluorescence spectroscopy. Compared with PSII dissolved in a micellar solution of n-dodecyl-β-d-maltoside (PSII-micelle), durability of PSII-DOPC and PSII-DOPG were enhanced by 1.3- and 1.5-fold, respectively. The activity and durability of PSII-EDOPC was significantly low. Lipid-dependent activity and durability were discussed in terms of kinetic parameters of V max and K m, and inhibition of the electron acceptor, phenyl-p-benzoquinone.
| Original language | English |
|---|---|
| Pages (from-to) | 3231-3241 |
| Number of pages | 11 |
| Journal | Research on Chemical Intermediates |
| Volume | 40 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Lipids bilayers
- Oxygen evolution activity
- Phosphatidylglycerol
- Photosynthesis
- Photosystem II
- Proteoliposome
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Durability of oxygen evolution of photosystem II incorporated into lipid bilayers. / Noji, Tomoyasu; Kondo, Masaharu; Kawakami, Keisuke; Shen, Jian-Ren; Nango, Mamoru; Dewa, Takehisa.
In: Research on Chemical Intermediates, Vol. 40, No. 9, 2014, p. 3231-3241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Durability of oxygen evolution of photosystem II incorporated into lipid bilayers
AU - Noji, Tomoyasu
AU - Kondo, Masaharu
AU - Kawakami, Keisuke
AU - Shen, Jian-Ren
AU - Nango, Mamoru
AU - Dewa, Takehisa
PY - 2014
Y1 - 2014
N2 - Photosystem II (PSII) has attracted a lot of attention for use in the construction of artificial photosynthetic materials due to its high activity of oxidation of water molecules. However, the robustness of PSII needs to be improved for in vitro application. In this study, we incorporated PSII (Thermosynechococcus vulcanus) into various phospholipid membranes to examine the activity and durability of oxygen evolution. PSII was incorporated into anionic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (PSII-DOPG), zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (PSII-DOPC), and cationic 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (PSII-EDOPC). Structural integrity of PSII was examined by absorption and fluorescence spectroscopy. Compared with PSII dissolved in a micellar solution of n-dodecyl-β-d-maltoside (PSII-micelle), durability of PSII-DOPC and PSII-DOPG were enhanced by 1.3- and 1.5-fold, respectively. The activity and durability of PSII-EDOPC was significantly low. Lipid-dependent activity and durability were discussed in terms of kinetic parameters of V max and K m, and inhibition of the electron acceptor, phenyl-p-benzoquinone.
AB - Photosystem II (PSII) has attracted a lot of attention for use in the construction of artificial photosynthetic materials due to its high activity of oxidation of water molecules. However, the robustness of PSII needs to be improved for in vitro application. In this study, we incorporated PSII (Thermosynechococcus vulcanus) into various phospholipid membranes to examine the activity and durability of oxygen evolution. PSII was incorporated into anionic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (PSII-DOPG), zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (PSII-DOPC), and cationic 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (PSII-EDOPC). Structural integrity of PSII was examined by absorption and fluorescence spectroscopy. Compared with PSII dissolved in a micellar solution of n-dodecyl-β-d-maltoside (PSII-micelle), durability of PSII-DOPC and PSII-DOPG were enhanced by 1.3- and 1.5-fold, respectively. The activity and durability of PSII-EDOPC was significantly low. Lipid-dependent activity and durability were discussed in terms of kinetic parameters of V max and K m, and inhibition of the electron acceptor, phenyl-p-benzoquinone.
KW - Lipids bilayers
KW - Oxygen evolution activity
KW - Phosphatidylglycerol
KW - Photosynthesis
KW - Photosystem II
KW - Proteoliposome
UR - http://www.scopus.com/inward/record.url?scp=84925493930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925493930&partnerID=8YFLogxK
U2 - 10.1007/s11164-014-1829-9
DO - 10.1007/s11164-014-1829-9
M3 - Article
VL - 40
SP - 3231
EP - 3241
JO - Research on Chemical Intermediates
JF - Research on Chemical Intermediates
SN - 0922-6168
IS - 9
ER -