Durability of oxygen evolution of photosystem II incorporated into lipid bilayers

Tomoyasu Noji, Masaharu Kondo, Keisuke Kawakami, Jian-Ren Shen, Mamoru Nango, Takehisa Dewa

Research output: Contribution to journalArticle

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Abstract

Photosystem II (PSII) has attracted a lot of attention for use in the construction of artificial photosynthetic materials due to its high activity of oxidation of water molecules. However, the robustness of PSII needs to be improved for in vitro application. In this study, we incorporated PSII (Thermosynechococcus vulcanus) into various phospholipid membranes to examine the activity and durability of oxygen evolution. PSII was incorporated into anionic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (PSII-DOPG), zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (PSII-DOPC), and cationic 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (PSII-EDOPC). Structural integrity of PSII was examined by absorption and fluorescence spectroscopy. Compared with PSII dissolved in a micellar solution of n-dodecyl-β-d-maltoside (PSII-micelle), durability of PSII-DOPC and PSII-DOPG were enhanced by 1.3- and 1.5-fold, respectively. The activity and durability of PSII-EDOPC was significantly low. Lipid-dependent activity and durability were discussed in terms of kinetic parameters of V max and K m, and inhibition of the electron acceptor, phenyl-p-benzoquinone.

Original languageEnglish
Pages (from-to)3231-3241
Number of pages11
JournalResearch on Chemical Intermediates
Volume40
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

Lipid bilayers
Photosystem II Protein Complex
Durability
Oxygen
Fluorescence spectroscopy
Micelles
Structural integrity
Absorption spectroscopy
Kinetic parameters
Phospholipids

Keywords

  • Lipids bilayers
  • Oxygen evolution activity
  • Phosphatidylglycerol
  • Photosynthesis
  • Photosystem II
  • Proteoliposome

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Durability of oxygen evolution of photosystem II incorporated into lipid bilayers. / Noji, Tomoyasu; Kondo, Masaharu; Kawakami, Keisuke; Shen, Jian-Ren; Nango, Mamoru; Dewa, Takehisa.

In: Research on Chemical Intermediates, Vol. 40, No. 9, 2014, p. 3231-3241.

Research output: Contribution to journalArticle

Noji, Tomoyasu ; Kondo, Masaharu ; Kawakami, Keisuke ; Shen, Jian-Ren ; Nango, Mamoru ; Dewa, Takehisa. / Durability of oxygen evolution of photosystem II incorporated into lipid bilayers. In: Research on Chemical Intermediates. 2014 ; Vol. 40, No. 9. pp. 3231-3241.
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