Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate

Shoji Sonoda, Xueyan Shi, Dunlun Song, Pei Liang, Xiwu Gao, Youjun Zhang, Jianhong Li, Yong Liu, Ming Li, Masaya Matsumura, Sachiyo Sanada-Morimura, Chieka Minakuchi, Toshiharu Tanaka, Tadashi Miyata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the field-collected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P.xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P.xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume48
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

acephate
Gene Duplication
Moths
Plutella xylostella
Acetylcholinesterase
acetylcholinesterase
Genes
mutation
Haplotypes
Mutation
haplotypes
genes
gene dosage
Gene Dosage
R Factors
gene duplication

Keywords

  • Acetylcholinesterase
  • Diamondback moth
  • Gene duplication
  • Mutation
  • Organophosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Insect Science
  • Biochemistry
  • Medicine(all)

Cite this

Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate. / Sonoda, Shoji; Shi, Xueyan; Song, Dunlun; Liang, Pei; Gao, Xiwu; Zhang, Youjun; Li, Jianhong; Liu, Yong; Li, Ming; Matsumura, Masaya; Sanada-Morimura, Sachiyo; Minakuchi, Chieka; Tanaka, Toshiharu; Miyata, Tadashi.

In: Insect Biochemistry and Molecular Biology, Vol. 48, No. 1, 2014, p. 83-90.

Research output: Contribution to journalArticle

Sonoda, S, Shi, X, Song, D, Liang, P, Gao, X, Zhang, Y, Li, J, Liu, Y, Li, M, Matsumura, M, Sanada-Morimura, S, Minakuchi, C, Tanaka, T & Miyata, T 2014, 'Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate', Insect Biochemistry and Molecular Biology, vol. 48, no. 1, pp. 83-90. https://doi.org/10.1016/j.ibmb.2014.02.008
Sonoda, Shoji ; Shi, Xueyan ; Song, Dunlun ; Liang, Pei ; Gao, Xiwu ; Zhang, Youjun ; Li, Jianhong ; Liu, Yong ; Li, Ming ; Matsumura, Masaya ; Sanada-Morimura, Sachiyo ; Minakuchi, Chieka ; Tanaka, Toshiharu ; Miyata, Tadashi. / Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate. In: Insect Biochemistry and Molecular Biology. 2014 ; Vol. 48, No. 1. pp. 83-90.
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