Duplication of a conditionally dispensable chromosome carrying pea pathogenicity (PEP) gene clusters in Nectria haematococca

Hamid S. Garmaroodi, Masatoki Taga

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A supernumerary chromosome called a conditionally dispensable chromosome (CDC) is essential for pathogenicity of Nectria haematococca on pea. Among several CDCs discovered in N. haematococca, the PDA1 CDC that harbors the pisatin demethylation gene PDA1 is one of the best-studied CDCs and serves as a model for plant-pathogenic fungi. Although the presence of multiple copies is usual for supernumerary chromosomes in other eukaryotes, this possibility has not been examined well for any CDCs in N. haematococca. In this study, we produced strains with multiple copies of the PDA1 CDC by protoplast fusion and analyzed dosage effects of this chromosome. Using multiple methods, including cytological chromosome counting and fluorescence in situ hybridization, the fusion products between two transformants derived from the same strain that bears a single PDA1 CDC were shown to contain two PDA1 CDCs from both transformants and estimated to be haploid resulting from the deletion of an extra set or sets of A chromosomes in the fused nuclei. In phenotype assays, dosage effects of PDA1 CDC in the fusion products were evident as increased virulence and homoserine-utilizing ability compared with the parents. In a separate fusion experiment, PDA1 CDC accumulated up to four copies in a haploid genome.

Original languageEnglish
Pages (from-to)1495-1504
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume20
Issue number12
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Nectria
Fusarium eumartii
Peas
Chromosomes
Multigene Family
multigene family
Virulence
peas
pathogenicity
Genes
chromosomes
Centers for Disease Control and Prevention (U.S.)
Fusion reactions
haploidy
Haploidy
dose response
pisatin
homoserine
protoplast fusion
Homoserine

Keywords

  • FISH
  • Fusarium solani
  • Germ tube burst method

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Duplication of a conditionally dispensable chromosome carrying pea pathogenicity (PEP) gene clusters in Nectria haematococca. / Garmaroodi, Hamid S.; Taga, Masatoki.

In: Molecular Plant-Microbe Interactions, Vol. 20, No. 12, 12.2007, p. 1495-1504.

Research output: Contribution to journalArticle

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