Duodenal follicular lymphoma: Comprehensive gene expression analysis with insights into pathogenesis

Katsuyoshi Takata, Motohiko Tanino, Daisuke Ennishi, Akira Tari, Yasuharu Sato, Hiroyuki Okada, Yoshinobu Maeda, Naoe Goto, Hiroshi Araki, Mai Harada, Midori Ando, Masaya Iwamuro, Mitsune Tanimoto, Kazuhide Yamamoto, Randy D. Gascoyne, Tadashi Yoshino

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Follicular lymphoma (FL) of the gastrointestinal tract, particularly duodenal follicular lymphoma (DFL), is a rare variant of FL with indolent clinical behavior, and this disease is included in the 2008 World Health Organization classification system. In contrast to nodal follicular lymphoma (NFL), DFL occurs most frequently in the second part of the duodenum, lacks follicular dendritic cell meshworks and has memory B-cell characteristics. However, its molecular pathogenesis is still unclear. In the present study, we examined 10 DFL, 18 NFL and 10 gastric MALT lymphoma samples using gene expression analysis. Quantitative RT-PCR experiments and immunohistochemical analysis for 72 formalin-fixed, paraffin-embedded tissues from an independent series, including 32 DFL, 19 gastric MALT lymphoma and 27 NFL samples, were performed for validation of microarray data. Gene expression profiles of the three lymphoma types were compared using 2918 differentially expressed genes (DEG) and results suggested that DFL shares characteristics of MALT lymphoma. Among these DEG, CCL20 and MAdCAM-1 were upregulated in DFL and MALT but downregulated in NFL. In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL. Quantitative RT-PCR and immunohistochemical studies demonstrated concordant results. Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT. We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.

Original languageEnglish
Pages (from-to)608-615
Number of pages8
JournalCancer Science
Volume105
Issue number5
DOIs
Publication statusPublished - May 2014

Keywords

  • CCL20
  • Duodenal follicular lymphoma
  • Gene expression profile
  • MALT lymphoma
  • MAdCAM-1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Takata, K., Tanino, M., Ennishi, D., Tari, A., Sato, Y., Okada, H., Maeda, Y., Goto, N., Araki, H., Harada, M., Ando, M., Iwamuro, M., Tanimoto, M., Yamamoto, K., Gascoyne, R. D., & Yoshino, T. (2014). Duodenal follicular lymphoma: Comprehensive gene expression analysis with insights into pathogenesis. Cancer Science, 105(5), 608-615. https://doi.org/10.1111/cas.12392