Drug susceptibility and beta-lactamase substrate profile of vibrio cholerae non-01

K. Tateda, K. Yamaguchi, Y. Ishii, K. Shimoguchi, N. Kusano, K. Sugahara, Nobuchika Kusano, S. Kohno, K. Hara

Research output: Contribution to journalArticle

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Abstract

Vibrio cholerae non-01 are organisms that are biochemically indistinguishable from V. cholerae but do not agglutinate in vibrio group 1 antiserum. Some of these strains have been known to produce a cholera-like toxin and cause dehydrating gastroenteritis in humans. Some reports state that more than 20% of V. cholerae isolated from blood are non-01 especially in patients with liver disease. We investigated drug susceptibility and resistance mechanisms to beta-lactam antibiotics in clinical (10 strains) and environmental isolates (80 strains) of V. cholerae non-01. Of the antibiotics tested, ceftizoxime and ofloxacin were extremely active against V. cholerae non -01: both antibiotics inhibited growth of all strains with 0.025 mg/1. None of the 10 strains of the clinical isolates was resistant to other antibiotics tested. On the other hand, 30 strains of the 80 environmental isolates (37.5%) were resistant to ampicillin (ABPC). All these strains showed MICs of ≥ 12.5 mg/1 and all produced beta-lactamase. In a substrate profile study of this enzyme, it was clarified that the beta-lactamase produced by V. cholerae non-01 was basically penicillinase. There is a possibility thit thus resistance to ABPC in environmental isolates of V. cholerae will spread to clinical isolates. We therefore need to be on our guard against the emergence of resistant strains in clinical V. cholerae non-01 infection.

Original languageEnglish
Pages (from-to)444-449
Number of pages6
JournalChemotherapy
Volume38
Issue number5
Publication statusPublished - 1990
Externally publishedYes

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Vibrio cholerae
beta-Lactamases
Pharmaceutical Preparations
Anti-Bacterial Agents
Ceftizoxime
Penicillinase
Ofloxacin
Vibrio
Cholera Toxin
Gastroenteritis
beta-Lactams
Ampicillin
Drug Resistance
Liver Diseases
Immune Sera
Enzymes
Growth
Infection

ASJC Scopus subject areas

  • Pharmacology

Cite this

Tateda, K., Yamaguchi, K., Ishii, Y., Shimoguchi, K., Kusano, N., Sugahara, K., ... Hara, K. (1990). Drug susceptibility and beta-lactamase substrate profile of vibrio cholerae non-01. Chemotherapy, 38(5), 444-449.

Drug susceptibility and beta-lactamase substrate profile of vibrio cholerae non-01. / Tateda, K.; Yamaguchi, K.; Ishii, Y.; Shimoguchi, K.; Kusano, N.; Sugahara, K.; Kusano, Nobuchika; Kohno, S.; Hara, K.

In: Chemotherapy, Vol. 38, No. 5, 1990, p. 444-449.

Research output: Contribution to journalArticle

Tateda, K, Yamaguchi, K, Ishii, Y, Shimoguchi, K, Kusano, N, Sugahara, K, Kusano, N, Kohno, S & Hara, K 1990, 'Drug susceptibility and beta-lactamase substrate profile of vibrio cholerae non-01', Chemotherapy, vol. 38, no. 5, pp. 444-449.
Tateda K, Yamaguchi K, Ishii Y, Shimoguchi K, Kusano N, Sugahara K et al. Drug susceptibility and beta-lactamase substrate profile of vibrio cholerae non-01. Chemotherapy. 1990;38(5):444-449.
Tateda, K. ; Yamaguchi, K. ; Ishii, Y. ; Shimoguchi, K. ; Kusano, N. ; Sugahara, K. ; Kusano, Nobuchika ; Kohno, S. ; Hara, K. / Drug susceptibility and beta-lactamase substrate profile of vibrio cholerae non-01. In: Chemotherapy. 1990 ; Vol. 38, No. 5. pp. 444-449.
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