Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants

Majid Mahdieh, Akbar Mostajeran, Tomoaki Horie, Maki Katsuhara

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Plasma membrane intrinsic proteins (PIPs), a type of aquaporins, mediate water transport in many plant species. In this study, we investigated the relationship between the functions of PIP-type water channels and water relations of tobacco plants (Nicotiana tabacum cv. Samsun) under drought stress. Drought stress treatments have led to reductions in the stomatal conductance, transpiration, water potential and turgor pressure in leaves, and also the sap flow rate and osmotic hydraulic conductance in roots. In contrast, leaf osmotic pressure was increased in response to drought stress. Interestingly, the accumulation of NtPIP1;1 and NtPIP2;1 transcripts was significantly decreased, but only that of the NtAQP1 transcript was increased under drought stress. Functional analysis using Xenopus laevis oocytes revealed that NtPIP2;1 shows marked water transport activity, but the activities of NtAQP1 and NtPIP1;1 are weak or almost negligible, respectively, when expressed alone. However, co-expression of NtPIP1;1 with NtPIP2;1 significantly enhanced water transport activity compared with that of NtPIP1;1- or NtPIP2;1-expressing oocytes, suggesting that these two aquaporins may function as a water channel, forming a heterotetramer. Heteromerization of NtPIP1;1 and NtPIP2;1 was also suggested by co-expression analyses of NtPIP1;1-GFP (green fluorescent protein) and NtPIP2;1 in Xenopus oocytes. Re-watering treatments recovered water relation parameters and the accumulation of the three NtPIP transcripts to levels similar to control conditions. These results suggest that NtPIP1;1 and NtPIP2;1 play an important role in water transport in roots, and that expression of NtPIP1;1 and NtPIP2;1 is down-regulated in order to reduce osmotic hydraulic conductance in the roots of tobacco plants under drought stress.

Original languageEnglish
Pages (from-to)801-813
Number of pages13
JournalPlant and Cell Physiology
Volume49
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint

Aquaporins
aquaporins
Droughts
Dehydration
membrane proteins
Nicotiana tabacum
blood proteins
Tobacco
Blood Proteins
Membrane Proteins
water stress
plasma membrane
Cell Membrane
Water
Genes
Oocytes
genes
water
root hydraulic conductivity
oocytes

Keywords

  • Aquaporin
  • Drought stress
  • Nicotiana tabacum
  • Water channel
  • Water relations

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants. / Mahdieh, Majid; Mostajeran, Akbar; Horie, Tomoaki; Katsuhara, Maki.

In: Plant and Cell Physiology, Vol. 49, No. 5, 05.2008, p. 801-813.

Research output: Contribution to journalArticle

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