Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems

Qing Xin Liu, Marek Jindra, Hitoshi Ueda, Yasushi Hiromi, Susumu Hirose

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

During gene activation, the effect of binding of transcription factors to cis-acting DNA sequences is transmitted to RNA polymerase by means of co-activators. Although co-activators contribute to the efficiency of transcription, their developmental roles are poorly understood. We used Drosophila to conduct molecular and genetic dissection of an evolutionarily conserved but unique co-activator, Multiprotein Bridging Factor 1 (MBF1), in a multicellular organism. Through immunoprecipitation, MBF1 was found to form a ternary complex including MBF1, TATA-binding protein (TBP) and the bZIP protein Tracheae Defective (TDF)/Apontic. We have isolated a Drosophila mutant that lacks the mbf1 gene in which no stable association between TBP and TDF is detectable, and transcription of a TDF-dependent reporter gene is reduced by 80%. Although the null mutants of mbf1 are viable, tdf becomes haploinsufficient in mbf1-deficient background, causing severe lesions in tracheae and the central nervous system, similar to those resulting from a complete loss of tdf function. These data demonstrate a crucial role of MBF1 in the development of tracheae and central nervous system.

Original languageEnglish
Pages (from-to)719-728
Number of pages10
JournalDevelopment
Volume130
Issue number4
DOIs
Publication statusPublished - Feb 2003
Externally publishedYes

Fingerprint

Trachea
Nervous System
Drosophila
TATA-Box Binding Protein
Central Nervous System
Basic-Leucine Zipper Transcription Factors
Multiprotein Complexes
DNA-Directed RNA Polymerases
Reporter Genes
Immunoprecipitation
Transcriptional Activation
Dissection
Molecular Biology
Transcription Factors
Genes

Keywords

  • CNS
  • Co-activator
  • Drosophila
  • MBF1
  • TDF
  • Trachea

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems. / Liu, Qing Xin; Jindra, Marek; Ueda, Hitoshi; Hiromi, Yasushi; Hirose, Susumu.

In: Development, Vol. 130, No. 4, 02.2003, p. 719-728.

Research output: Contribution to journalArticle

Liu, Qing Xin ; Jindra, Marek ; Ueda, Hitoshi ; Hiromi, Yasushi ; Hirose, Susumu. / Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems. In: Development. 2003 ; Vol. 130, No. 4. pp. 719-728.
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