Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor

Tsukasa Shimojima, Masahiro Okada, Takahiro Nakayama, Hitoshi Ueda, Katsuya Okawa, Akihiro Iwamatsu, Hiroshi Handa, Susumu Hirose

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-directed chromatin remodeling. Moreover, genetic interactions between Trithorax-like encoding GAGA factor and spt16 implicate the GAGA factor-FACT complex in expression of Hox genes Ultrabithorax, Sex combs reduced, and Abdominal-B. Chromatin immunoprecipitation experiments indicated the presence of the GAGA factor-FACT complex in the regulatory regions of Ultrabithorax and Abdominal-B. These data illustrate a crucial role of FACT in the modulation of chromatin structure for the regulation of gene expression.

Original languageEnglish
Pages (from-to)1605-1616
Number of pages12
JournalGenes and Development
Volume17
Issue number13
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

Fingerprint

Homeobox Genes
Drosophila
Chromatin
Gene Expression
Chromatin Assembly and Disassembly
Comb and Wattles
Nucleosomes
Chromatin Immunoprecipitation
Nucleic Acid Regulatory Sequences
Gene Expression Regulation
Binding Sites

Keywords

  • Chromatin
  • Drosophila
  • FACT
  • GAGA factor
  • Hox gene

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor. / Shimojima, Tsukasa; Okada, Masahiro; Nakayama, Takahiro; Ueda, Hitoshi; Okawa, Katsuya; Iwamatsu, Akihiro; Handa, Hiroshi; Hirose, Susumu.

In: Genes and Development, Vol. 17, No. 13, 01.07.2003, p. 1605-1616.

Research output: Contribution to journalArticle

Shimojima, T, Okada, M, Nakayama, T, Ueda, H, Okawa, K, Iwamatsu, A, Handa, H & Hirose, S 2003, 'Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor', Genes and Development, vol. 17, no. 13, pp. 1605-1616. https://doi.org/10.1101/gad.1086803
Shimojima, Tsukasa ; Okada, Masahiro ; Nakayama, Takahiro ; Ueda, Hitoshi ; Okawa, Katsuya ; Iwamatsu, Akihiro ; Handa, Hiroshi ; Hirose, Susumu. / Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor. In: Genes and Development. 2003 ; Vol. 17, No. 13. pp. 1605-1616.
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