Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway

Yasuo Agawa, Moustafa Sarhan, Yuji Kageyama, Kazutaka Akagi, Masayoshi Takai, Kazuya Hashiyama, Tadashi Wada, Hiroshi Handa, Akihiro Iwamatsu, Susumu Hirose, Hitoshi Ueda

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36 Citations (Scopus)

Abstract

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-f1. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.

Original languageEnglish
Pages (from-to)8739-8747
Number of pages9
JournalMolecular and Cellular Biology
Volume27
Issue number24
DOIs
Publication statusPublished - Dec 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Agawa, Y., Sarhan, M., Kageyama, Y., Akagi, K., Takai, M., Hashiyama, K., Wada, T., Handa, H., Iwamatsu, A., Hirose, S., & Ueda, H. (2007). Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway. Molecular and Cellular Biology, 27(24), 8739-8747. https://doi.org/10.1128/MCB.01304-07