Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume

Keiichi Mochida, Tetsuya Sakurai, Hikaru Seki, Takuhiro Yoshida, Kotaro Takahagi, Satoru Sawai, Hiroshi Uchiyama, Toshiya Muranaka, Kazuki Saito

    Research output: Contribution to journalArticlepeer-review

    82 Citations (Scopus)

    Abstract

    Chinese liquorice/licorice (Glycyrrhiza uralensis) is a leguminous plant species whose roots and rhizomes have been widely used as a herbal medicine and natural sweetener. Whole-genome sequencing is essential for gene discovery studies and molecular breeding in liquorice. Here, we report a draft assembly of the approximately 379-Mb whole-genome sequence of strain 308-19 of G. uralensis; this assembly contains 34 445 predicted protein-coding genes. Comparative analyses suggested well-conserved genomic components and collinearity of gene loci (synteny) between the genome of liquorice and those of other legumes such as Medicago and chickpea. We observed that three genes involved in isoflavonoid biosynthesis, namely, 2-hydroxyisoflavanone synthase (CYP93C), 2,7,4′-trihydroxyisoflavanone 4′-O-methyltransferase/isoflavone 4′-O-methyltransferase (HI4OMT) and isoflavone-7-O-methyltransferase (7-IOMT) formed a cluster on the scaffold of the liquorice genome and showed conserved microsynteny with Medicago and chickpea. Based on the liquorice genome annotation, we predicted genes in the P450 and UDP-dependent glycosyltransferase (UGT) superfamilies, some of which are involved in triterpenoid saponin biosynthesis, and characterised their gene expression with the reference genome sequence. The genome sequencing and its annotations provide an essential resource for liquorice improvement through molecular breeding and the discovery of useful genes for engineering bioactive components through synthetic biology approaches.

    Original languageEnglish
    Pages (from-to)181-194
    Number of pages14
    JournalPlant Journal
    Volume89
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 2017

    Keywords

    • Glycyrrhiza uralensis
    • P450
    • PRJDB3943
    • UDP-dependent glycosyltransferases
    • flavonoid
    • genome sequence
    • liquorice/licorice
    • synteny

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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