Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume

Keiichi Mochida, Tetsuya Sakurai, Hikaru Seki, Takuhiro Yoshida, Kotaro Takahagi, Satoru Sawai, Hiroshi Uchiyama, Toshiya Muranaka, Kazuki Saito

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Chinese liquorice/licorice (Glycyrrhiza uralensis) is a leguminous plant species whose roots and rhizomes have been widely used as a herbal medicine and natural sweetener. Whole-genome sequencing is essential for gene discovery studies and molecular breeding in liquorice. Here, we report a draft assembly of the approximately 379-Mb whole-genome sequence of strain 308-19 of G. uralensis; this assembly contains 34 445 predicted protein-coding genes. Comparative analyses suggested well-conserved genomic components and collinearity of gene loci (synteny) between the genome of liquorice and those of other legumes such as Medicago and chickpea. We observed that three genes involved in isoflavonoid biosynthesis, namely, 2-hydroxyisoflavanone synthase (CYP93C), 2,7,4′-trihydroxyisoflavanone 4′-O-methyltransferase/isoflavone 4′-O-methyltransferase (HI4OMT) and isoflavone-7-O-methyltransferase (7-IOMT) formed a cluster on the scaffold of the liquorice genome and showed conserved microsynteny with Medicago and chickpea. Based on the liquorice genome annotation, we predicted genes in the P450 and UDP-dependent glycosyltransferase (UGT) superfamilies, some of which are involved in triterpenoid saponin biosynthesis, and characterised their gene expression with the reference genome sequence. The genome sequencing and its annotations provide an essential resource for liquorice improvement through molecular breeding and the discovery of useful genes for engineering bioactive components through synthetic biology approaches.

Original languageEnglish
Pages (from-to)181-194
Number of pages14
JournalPlant Journal
Volume89
Issue number2
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Glycyrrhiza uralensis
genome assembly
licorice
Glycyrrhiza
Fabaceae
legumes
Genome
genome
methyltransferases
DNA Shuffling
Cicer
Medicago
Methyltransferases
Genetic Association Studies
isoflavones
genes
biosynthesis
Synthetic Biology
synthetic biology
Gene Components

Keywords

  • flavonoid
  • genome sequence
  • Glycyrrhiza uralensis
  • liquorice/licorice
  • P450
  • PRJDB3943
  • synteny
  • UDP-dependent glycosyltransferases

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Mochida, K., Sakurai, T., Seki, H., Yoshida, T., Takahagi, K., Sawai, S., ... Saito, K. (2017). Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume. Plant Journal, 89(2), 181-194. https://doi.org/10.1111/tpj.13385

Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume. / Mochida, Keiichi; Sakurai, Tetsuya; Seki, Hikaru; Yoshida, Takuhiro; Takahagi, Kotaro; Sawai, Satoru; Uchiyama, Hiroshi; Muranaka, Toshiya; Saito, Kazuki.

In: Plant Journal, Vol. 89, No. 2, 01.01.2017, p. 181-194.

Research output: Contribution to journalArticle

Mochida, K, Sakurai, T, Seki, H, Yoshida, T, Takahagi, K, Sawai, S, Uchiyama, H, Muranaka, T & Saito, K 2017, 'Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume', Plant Journal, vol. 89, no. 2, pp. 181-194. https://doi.org/10.1111/tpj.13385
Mochida, Keiichi ; Sakurai, Tetsuya ; Seki, Hikaru ; Yoshida, Takuhiro ; Takahagi, Kotaro ; Sawai, Satoru ; Uchiyama, Hiroshi ; Muranaka, Toshiya ; Saito, Kazuki. / Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume. In: Plant Journal. 2017 ; Vol. 89, No. 2. pp. 181-194.
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