Downregulation of TESTIN and its association with cancer history and a tendency toward poor survival in head and neck squamous cell carcinoma

Esra Gunduz, Mehmet Gunduz, Levent Beder, Hitoshi Nagatsuka, Kunihiro Fukushima, Recep Sutcu, Namik Delibas, Noboru Yamanaka, Kenji Shimizu, Noriyuki Nagai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To examine the role of TESTIN as a candidate tumor suppressor gene in head and neck carcinogenesis. Design: Mutation and messenger RNA (mRNA) expression analyses. Setting: Academic research. Patients: Paired normal and tumor samples were obtained from 38 patients with primary head and neck squa- mous cell carcinoma. Main Outcome Measures: Analysis and comparison of TESTIN gene mRNA expression and its relationship to clinicopathologic variables. Results: Mutation analysis showed a nucleotide and amino acid change in 6 of the 38 tumor samples (16.0%). Semiquantitative mRNA expression analysis of TESTIN revealed a decreased expression in approximately 50% of the tumors compared with their matched normal controls. Interestingly, comparison of clinicopathologic vari ables to mRNA expression status of TESTIN revealed a significant difference in terms of cancer history (P = .03). Moreover, a higher smoking ratio and a family cancer history were also associated with downregulation of TESTIN, although the difference was not statistically significant (P =.43 and P =.16, respectively). Kaplan-Meier survival analysis demonstrated a worse survival rate among the patients with low TESTIN expression compared with the patients with normal-high TESTIN expression. Conclusions: Our findings suggest that inactivation of TESTIN is involved in head and neck carcinogenesis through its downregulation. Further studies in various human cancer tissues using a large sample size and in vitro functional studies as well as clinical comparison research studies would give us a better evaluation of TESTINs role and its possible future application in molecular diagnosis and treatment of different cancer types, including head and neck squamous cell carcinoma.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume135
Issue number3
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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