Abstract
Objective: To examine the role of TESTIN as a candidate tumor suppressor gene in head and neck carcinogenesis. Design: Mutation and messenger RNA (mRNA) expression analyses. Setting: Academic research. Patients: Paired normal and tumor samples were obtained from 38 patients with primary head and neck squa- mous cell carcinoma. Main Outcome Measures: Analysis and comparison of TESTIN gene mRNA expression and its relationship to clinicopathologic variables. Results: Mutation analysis showed a nucleotide and amino acid change in 6 of the 38 tumor samples (16.0%). Semiquantitative mRNA expression analysis of TESTIN revealed a decreased expression in approximately 50% of the tumors compared with their matched normal controls. Interestingly, comparison of clinicopathologic vari ables to mRNA expression status of TESTIN revealed a significant difference in terms of cancer history (P = .03). Moreover, a higher smoking ratio and a family cancer history were also associated with downregulation of TESTIN, although the difference was not statistically significant (P =.43 and P =.16, respectively). Kaplan-Meier survival analysis demonstrated a worse survival rate among the patients with low TESTIN expression compared with the patients with normal-high TESTIN expression. Conclusions: Our findings suggest that inactivation of TESTIN is involved in head and neck carcinogenesis through its downregulation. Further studies in various human cancer tissues using a large sample size and in vitro functional studies as well as clinical comparison research studies would give us a better evaluation of TESTINs role and its possible future application in molecular diagnosis and treatment of different cancer types, including head and neck squamous cell carcinoma.
| Original language | English |
|---|---|
| Pages (from-to) | 254-260 |
| Number of pages | 7 |
| Journal | Archives of Otolaryngology - Head and Neck Surgery |
| Volume | 135 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Otorhinolaryngology
- Surgery
Cite this
Downregulation of TESTIN and its association with cancer history and a tendency toward poor survival in head and neck squamous cell carcinoma. / Gunduz, Esra; Gunduz, Mehmet; Beder, Levent; Nagatsuka, Hitoshi; Fukushima, Kunihiro; Sutcu, Recep; Delibas, Namik; Yamanaka, Noboru; Shimizu, Kenji; Nagai, Noriyuki.
In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 135, No. 3, 03.2009, p. 254-260.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Downregulation of TESTIN and its association with cancer history and a tendency toward poor survival in head and neck squamous cell carcinoma
AU - Gunduz, Esra
AU - Gunduz, Mehmet
AU - Beder, Levent
AU - Nagatsuka, Hitoshi
AU - Fukushima, Kunihiro
AU - Sutcu, Recep
AU - Delibas, Namik
AU - Yamanaka, Noboru
AU - Shimizu, Kenji
AU - Nagai, Noriyuki
PY - 2009/3
Y1 - 2009/3
N2 - Objective: To examine the role of TESTIN as a candidate tumor suppressor gene in head and neck carcinogenesis. Design: Mutation and messenger RNA (mRNA) expression analyses. Setting: Academic research. Patients: Paired normal and tumor samples were obtained from 38 patients with primary head and neck squa- mous cell carcinoma. Main Outcome Measures: Analysis and comparison of TESTIN gene mRNA expression and its relationship to clinicopathologic variables. Results: Mutation analysis showed a nucleotide and amino acid change in 6 of the 38 tumor samples (16.0%). Semiquantitative mRNA expression analysis of TESTIN revealed a decreased expression in approximately 50% of the tumors compared with their matched normal controls. Interestingly, comparison of clinicopathologic vari ables to mRNA expression status of TESTIN revealed a significant difference in terms of cancer history (P = .03). Moreover, a higher smoking ratio and a family cancer history were also associated with downregulation of TESTIN, although the difference was not statistically significant (P =.43 and P =.16, respectively). Kaplan-Meier survival analysis demonstrated a worse survival rate among the patients with low TESTIN expression compared with the patients with normal-high TESTIN expression. Conclusions: Our findings suggest that inactivation of TESTIN is involved in head and neck carcinogenesis through its downregulation. Further studies in various human cancer tissues using a large sample size and in vitro functional studies as well as clinical comparison research studies would give us a better evaluation of TESTINs role and its possible future application in molecular diagnosis and treatment of different cancer types, including head and neck squamous cell carcinoma.
AB - Objective: To examine the role of TESTIN as a candidate tumor suppressor gene in head and neck carcinogenesis. Design: Mutation and messenger RNA (mRNA) expression analyses. Setting: Academic research. Patients: Paired normal and tumor samples were obtained from 38 patients with primary head and neck squa- mous cell carcinoma. Main Outcome Measures: Analysis and comparison of TESTIN gene mRNA expression and its relationship to clinicopathologic variables. Results: Mutation analysis showed a nucleotide and amino acid change in 6 of the 38 tumor samples (16.0%). Semiquantitative mRNA expression analysis of TESTIN revealed a decreased expression in approximately 50% of the tumors compared with their matched normal controls. Interestingly, comparison of clinicopathologic vari ables to mRNA expression status of TESTIN revealed a significant difference in terms of cancer history (P = .03). Moreover, a higher smoking ratio and a family cancer history were also associated with downregulation of TESTIN, although the difference was not statistically significant (P =.43 and P =.16, respectively). Kaplan-Meier survival analysis demonstrated a worse survival rate among the patients with low TESTIN expression compared with the patients with normal-high TESTIN expression. Conclusions: Our findings suggest that inactivation of TESTIN is involved in head and neck carcinogenesis through its downregulation. Further studies in various human cancer tissues using a large sample size and in vitro functional studies as well as clinical comparison research studies would give us a better evaluation of TESTINs role and its possible future application in molecular diagnosis and treatment of different cancer types, including head and neck squamous cell carcinoma.
UR - http://www.scopus.com/inward/record.url?scp=63049131849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63049131849&partnerID=8YFLogxK
U2 - 10.1001/archoto.2008.560
DO - 10.1001/archoto.2008.560
M3 - Article
C2 - 19289703
AN - SCOPUS:63049131849
VL - 135
SP - 254
EP - 260
JO - JAMA Otolaryngology - Head and Neck Surgery
JF - JAMA Otolaryngology - Head and Neck Surgery
SN - 2168-6181
IS - 3
ER -