DOUBLE STAINING BY VAPORIZED OSMIUM TETROXIDE-HYDRAZINE HYDRATE OF BIOLOGICAL SPECIMENS FOR NON-COATED SCANNING ELECTRON MICROSCOPY.

Takuro Murakami, Akira Kubotsu, Aiji Ohtsuka, Shinji Akita, Yamamoto Kazuhide, Albert L. Jones

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sufficient electron conductivity was obtained in biological specimens following double staining with vaporized osmium tetroxide and hydrazine hydrate for non-gold coated scanning electron microscopic observations. No charging was noted at 25 kV acceleration voltage in rat kidney, liver or other specimens. The foot processes of the glomerular podocytes and micropores of the glomerular endothelium as well as the intracellular endoplasmic recticulum of liver cells were particularly well visualized without the addition of evaporated metal. Repeating this vapor phase osmication method produced even higher contrast at high magnifications compared to the single vaporized osmication method.

Original languageEnglish
Pages (from-to)459-464
Number of pages6
JournalScanning Electron Microscopy
Issue numberpt 1
Publication statusPublished - 1982

Fingerprint

hydrazine
Osmium Tetroxide
Osmium
Hydrazine
Hydrates
Liver
Electron Scanning Microscopy
Electrons
Staining and Labeling
Podocytes
Scanning electron microscopy
Endothelium
Rats
Foot
Metals
Vapors
Scanning
Kidney
Electric potential

ASJC Scopus subject areas

  • Biophysics
  • Control and Systems Engineering

Cite this

DOUBLE STAINING BY VAPORIZED OSMIUM TETROXIDE-HYDRAZINE HYDRATE OF BIOLOGICAL SPECIMENS FOR NON-COATED SCANNING ELECTRON MICROSCOPY. / Murakami, Takuro; Kubotsu, Akira; Ohtsuka, Aiji; Akita, Shinji; Kazuhide, Yamamoto; Jones, Albert L.

In: Scanning Electron Microscopy, No. pt 1, 1982, p. 459-464.

Research output: Contribution to journalArticle

Murakami, Takuro ; Kubotsu, Akira ; Ohtsuka, Aiji ; Akita, Shinji ; Kazuhide, Yamamoto ; Jones, Albert L. / DOUBLE STAINING BY VAPORIZED OSMIUM TETROXIDE-HYDRAZINE HYDRATE OF BIOLOGICAL SPECIMENS FOR NON-COATED SCANNING ELECTRON MICROSCOPY. In: Scanning Electron Microscopy. 1982 ; No. pt 1. pp. 459-464.
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