Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana: Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit

Yoshinobu Kimura, Yuki Takeoka, Masami Inoue, Megumi Maeda, Kazuhito Fujiyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Endo-β-N-acetylglucosaminidase (ENGase) is involved in the production of high-mannose type free Nglycans during plant development and fruit maturation. In a previous study (K. Nakamura et al. Biosci. Biotechnol. Biochem., 73, 461-464 (2009)), we identified the tomato ENGase gene and found that gene expression remained relatively constant. In the present study, we constructed an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed. The mutant showed no ENGase activity, but produced high-mannose type free N-glycans carrying the N,N'-acetylchitobiosyl unit that is produced by peptide:N-glycanase, indicating that both these genes encode Arabidopsis ENGase.

Original languageEnglish
Pages (from-to)1019-1021
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number5
DOIs
Publication statusPublished - Jun 6 2011

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Keywords

  • Arabidopsis thaliana
  • De-N-glycosylation
  • Endo-β-N-acetylglucosaminidase
  • Free Nglycans

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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