Abstract
Endo-β-N-acetylglucosaminidase (ENGase) is involved in the production of high-mannose type free Nglycans during plant development and fruit maturation. In a previous study (K. Nakamura et al. Biosci. Biotechnol. Biochem., 73, 461-464 (2009)), we identified the tomato ENGase gene and found that gene expression remained relatively constant. In the present study, we constructed an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed. The mutant showed no ENGase activity, but produced high-mannose type free N-glycans carrying the N,N'-acetylchitobiosyl unit that is produced by peptide:N-glycanase, indicating that both these genes encode Arabidopsis ENGase.
| Original language | English |
|---|---|
| Pages (from-to) | 1019-1021 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 75 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2011 |
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Keywords
- Arabidopsis thaliana
- De-N-glycosylation
- Endo-β-N-acetylglucosaminidase
- Free Nglycans
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Applied Microbiology and Biotechnology
- Analytical Chemistry
- Organic Chemistry
Cite this
Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana : Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit. / Kimura, Yoshinobu; Takeoka, Yuki; Inoue, Masami; Maeda, Megumi; Fujiyama, Kazuhito.
In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 5, 2011, p. 1019-1021.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana
T2 - Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit
AU - Kimura, Yoshinobu
AU - Takeoka, Yuki
AU - Inoue, Masami
AU - Maeda, Megumi
AU - Fujiyama, Kazuhito
PY - 2011
Y1 - 2011
N2 - Endo-β-N-acetylglucosaminidase (ENGase) is involved in the production of high-mannose type free Nglycans during plant development and fruit maturation. In a previous study (K. Nakamura et al. Biosci. Biotechnol. Biochem., 73, 461-464 (2009)), we identified the tomato ENGase gene and found that gene expression remained relatively constant. In the present study, we constructed an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed. The mutant showed no ENGase activity, but produced high-mannose type free N-glycans carrying the N,N'-acetylchitobiosyl unit that is produced by peptide:N-glycanase, indicating that both these genes encode Arabidopsis ENGase.
AB - Endo-β-N-acetylglucosaminidase (ENGase) is involved in the production of high-mannose type free Nglycans during plant development and fruit maturation. In a previous study (K. Nakamura et al. Biosci. Biotechnol. Biochem., 73, 461-464 (2009)), we identified the tomato ENGase gene and found that gene expression remained relatively constant. In the present study, we constructed an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed. The mutant showed no ENGase activity, but produced high-mannose type free N-glycans carrying the N,N'-acetylchitobiosyl unit that is produced by peptide:N-glycanase, indicating that both these genes encode Arabidopsis ENGase.
KW - Arabidopsis thaliana
KW - De-N-glycosylation
KW - Endo-β-N-acetylglucosaminidase
KW - Free Nglycans
UR - http://www.scopus.com/inward/record.url?scp=79957843060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957843060&partnerID=8YFLogxK
U2 - 10.1271/bbb.110148
DO - 10.1271/bbb.110148
M3 - Article
C2 - 21597164
AN - SCOPUS:79957843060
VL - 75
SP - 1019
EP - 1021
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 5
ER -