Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana: Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit

Yoshinobu Kimura; Yuki Takeoka; Masami Inoue; Megumi Maeda; Kazuhito Fujiyama

doi:10.1271/bbb.110148

Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana: Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit

Yoshinobu Kimura, Yuki Takeoka, Masami Inoue, Megumi Maeda, Kazuhito Fujiyama

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

Endo-β-N-acetylglucosaminidase (ENGase) is involved in the production of high-mannose type free Nglycans during plant development and fruit maturation. In a previous study (K. Nakamura et al. Biosci. Biotechnol. Biochem., 73, 461-464 (2009)), we identified the tomato ENGase gene and found that gene expression remained relatively constant. In the present study, we constructed an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed. The mutant showed no ENGase activity, but produced high-mannose type free N-glycans carrying the N,N'-acetylchitobiosyl unit that is produced by peptide:N-glycanase, indicating that both these genes encode Arabidopsis ENGase.

Original language	English
Pages (from-to)	1019-1021
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	75
Issue number	5
DOIs	https://doi.org/10.1271/bbb.110148
Publication status	Published - Jun 6 2011

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Keywords

Arabidopsis thaliana
De-N-glycosylation
Endo-β-N-acetylglucosaminidase
Free Nglycans

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Cite this

Kimura, Y., Takeoka, Y., Inoue, M., Maeda, M., & Fujiyama, K. (2011). Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana: Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit. Bioscience, Biotechnology and Biochemistry, 75(5), 1019-1021. https://doi.org/10.1271/bbb.110148

Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana : Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit. / Kimura, Yoshinobu; Takeoka, Yuki; Inoue, Masami; Maeda, Megumi; Fujiyama, Kazuhito.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 5, 06.06.2011, p. 1019-1021.

Research output: Contribution to journal › Article

Kimura, Y, Takeoka, Y, Inoue, M, Maeda, M & Fujiyama, K 2011, 'Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana: Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit', Bioscience, Biotechnology and Biochemistry, vol. 75, no. 5, pp. 1019-1021. https://doi.org/10.1271/bbb.110148

Kimura Y, Takeoka Y, Inoue M, Maeda M, Fujiyama K. Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana: Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit. Bioscience, Biotechnology and Biochemistry. 2011 Jun 6;75(5):1019-1021. https://doi.org/10.1271/bbb.110148

Kimura, Yoshinobu ; Takeoka, Yuki ; Inoue, Masami ; Maeda, Megumi ; Fujiyama, Kazuhito. / Double-knockout of putative endo-β-N-Acetylglucosaminidase (ENGase) genes in arabidopsis thaliana : Loss of ENGase activity induced accumulation of high-mannose type free N-Glycans Bearing N,N'-Acetylchitobiosyl unit. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 5. pp. 1019-1021.

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10.1271/bbb.110148