Domain movement in gelsolin: A calcium-activated switch

Robert C. Robinson, Marisan Mejillano, Vincent P. Le, Leslie D. Burtnick, Helen L. Yin, Senyon Choe

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

The actin-binding protein gelsolin is involved in remodeling the actin cytoskeleton during growth-factor signaling, apoptosis, cytokinesis, and cell movement. Calcium-activated gelsolin severs and caps actin filaments. The 3.4 angstrom x-ray structure of the carboxyl-terminal half of gelsolin (G4-G6) in complex with actin reveals the basis for gelsolin activation. Calcium binding induces a conformational rearrangement in which domain G6 is flipped over and translated by about 40 angstroms relative to G4 and G5. The structural reorganization tears apart the continuous β sheet core of G4 and G6. This exposes the actin-binding site on G4, enabling severing and capping of actin filaments to proceed.

Original languageEnglish
Pages (from-to)1939-1942
Number of pages4
JournalScience
Volume286
Issue number5446
DOIs
Publication statusPublished - Dec 3 1999
Externally publishedYes

Fingerprint

Gelsolin
Actin Cytoskeleton
Calcium
Actins
Microfilament Proteins
Cytokinesis
Tears
Cell Movement
Intercellular Signaling Peptides and Proteins
Binding Sites
X-Rays
Apoptosis

ASJC Scopus subject areas

  • General

Cite this

Robinson, R. C., Mejillano, M., Le, V. P., Burtnick, L. D., Yin, H. L., & Choe, S. (1999). Domain movement in gelsolin: A calcium-activated switch. Science, 286(5446), 1939-1942. https://doi.org/10.1126/science.286.5446.1939

Domain movement in gelsolin : A calcium-activated switch. / Robinson, Robert C.; Mejillano, Marisan; Le, Vincent P.; Burtnick, Leslie D.; Yin, Helen L.; Choe, Senyon.

In: Science, Vol. 286, No. 5446, 03.12.1999, p. 1939-1942.

Research output: Contribution to journalArticle

Robinson, RC, Mejillano, M, Le, VP, Burtnick, LD, Yin, HL & Choe, S 1999, 'Domain movement in gelsolin: A calcium-activated switch', Science, vol. 286, no. 5446, pp. 1939-1942. https://doi.org/10.1126/science.286.5446.1939
Robinson RC, Mejillano M, Le VP, Burtnick LD, Yin HL, Choe S. Domain movement in gelsolin: A calcium-activated switch. Science. 1999 Dec 3;286(5446):1939-1942. https://doi.org/10.1126/science.286.5446.1939
Robinson, Robert C. ; Mejillano, Marisan ; Le, Vincent P. ; Burtnick, Leslie D. ; Yin, Helen L. ; Choe, Senyon. / Domain movement in gelsolin : A calcium-activated switch. In: Science. 1999 ; Vol. 286, No. 5446. pp. 1939-1942.
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