Docosahexaenoic acid induces apoptosis via the bax-independent pathway in HL-60 cells

Yoshie Miura; Keigo Takahara; Yoshiyuki Murata; Kozo Utsumi; Mikiro Tada; Kyoya Takahata

doi:10.1271/bbb.68.2415

Docosahexaenoic acid induces apoptosis via the bax-independent pathway in HL-60 cells

Yoshie Miura, Keigo Takahara, Yoshiyuki Murata, Kozo Utsumi, Mikiro Tada, Kyoya Takahata

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We attempted to determine whether docosahexaenoic acid (DHA)-induced apoptosis is mediated via the Bax-mediated pathway in human myeloid leukemia HL-60 cells. DHA-induced apoptosis was confirmed by morphological analysis and caspase-3 activation. But, cyclosporin A (CsA), an inhibitor of mitochondrial permeability transition (MPT), did not inhibit DHA-induced Bax translocation to mitochondria or caspase-3 activation. These data suggest that DHA can induce apoptosis via the Bax-independent pathway.

Original language	English
Pages (from-to)	2415-2417
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	68
Issue number	11
DOIs	https://doi.org/10.1271/bbb.68.2415
Publication status	Published - Nov 1 2004

Keywords

Apoptosis
Bax
Caspase-3
Docosahexaenoic acid (DHA)
HL-60 cells

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Docosahexaenoic acid induces apoptosis via the bax-independent pathway in HL-60 cells",

abstract = "We attempted to determine whether docosahexaenoic acid (DHA)-induced apoptosis is mediated via the Bax-mediated pathway in human myeloid leukemia HL-60 cells. DHA-induced apoptosis was confirmed by morphological analysis and caspase-3 activation. But, cyclosporin A (CsA), an inhibitor of mitochondrial permeability transition (MPT), did not inhibit DHA-induced Bax translocation to mitochondria or caspase-3 activation. These data suggest that DHA can induce apoptosis via the Bax-independent pathway.",

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AU - Takahara, Keigo

AU - Murata, Yoshiyuki

AU - Utsumi, Kozo

AU - Tada, Mikiro

AU - Takahata, Kyoya

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N2 - We attempted to determine whether docosahexaenoic acid (DHA)-induced apoptosis is mediated via the Bax-mediated pathway in human myeloid leukemia HL-60 cells. DHA-induced apoptosis was confirmed by morphological analysis and caspase-3 activation. But, cyclosporin A (CsA), an inhibitor of mitochondrial permeability transition (MPT), did not inhibit DHA-induced Bax translocation to mitochondria or caspase-3 activation. These data suggest that DHA can induce apoptosis via the Bax-independent pathway.

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KW - Caspase-3

KW - Docosahexaenoic acid (DHA)

KW - HL-60 cells

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