DNA strand breaks induced by ionizing radiation on Rubrobacter radiotolerans, an extremely radioresistant bacterium

Hiroaki Terato, Mutsumi Kobayashi, Osamu Yamamoto, Hiroshi Ide

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rubrobacter radiotoIerans is the most radioresistant bacterium showing 16kGy as D37 against gamma-rays. However mechanisms of the radioresistance have been still unclear. To clarify the post-irradiating events in the cell, we investigated DNA strand breaks which is the most major lesion induced by ionizing radiation. The neutral sucrose density gradient centrifugation analysis showed that size of the chromosomal DNA gradually decreased with irradiation dose. However, the frequency of DNA strand breaks after ionizing irradiation was significantly lower than those reported for other eubacteria. The reduced DNA sizes were not recovered during the postirradiating cultivation. These results suggest that in this organism DNA protection from damage is mainly involved in the radioresistance, but not DNA repair activities.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalMicrobiological Research
Volume154
Issue number2
DOIs
Publication statusPublished - Sep 1999
Externally publishedYes

Keywords

  • DNA damage
  • Free radical
  • Radiation
  • Radioresistance
  • Rubrobacter radiotolerans

ASJC Scopus subject areas

  • Microbiology

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