Abstract
Gassericin A, produced by Lactobacillus gasseri LA39, is a hydrophobic circular bacteriocin. The DNA region surrounding the gassericin A structural gene, gaaA, was sequenced, and seven open reading frames (ORFs) of 3.5 kbp (gaaBCADITE) were found with possible functions in gassericin A production, secretion, and immunity. The deduced products of the five consecutive ORFs gaaADITE have homology to those of genes involved in butyrivibriocin AR10 production, although the genetic arrangements are different in the two circular bacteriocin genes. Gaal is a small, positively charged hydrophobic peptide of 53 amino acids containing a putative transmembrane segment. Heterologous expression and homologous expression of Gaal in Lactococcus lactis subsp. cremoris MG1363 and L. gasseri JCM1131T, respectively, were studied. Gaal-expressing strains exhibited at least sevenfold-higher resistance to gassericin A than corresponding control strains, indicating that goal encodes an immunity peptide for gassericin A. Comparison of Gaal to peptides with similar characteristics found in the circular bacteriocin gene loci is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1324-1330 |
| Number of pages | 7 |
| Journal | Applied and Environmental Microbiology |
| Volume | 75 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Food Science
- Biotechnology
- Ecology
Cite this
DNA sequencing and homologous expression of a small peptide conferring immunity to gassericin a, a circular bacteriocin a produced by lactobacillus gasseri LA39. / Kawai, Yasushi; Kusnadi, Joni; Kemperman, Rober; Kok, Jan; Ito, Yoshiyuki; Endo, Mikiko; Arakawa, Kensuke; Uchida, Hideaki; Nishimura, Junko; Kitazawa, Haruki; Saito, Tadao.
In: Applied and Environmental Microbiology, Vol. 75, No. 5, 03.2009, p. 1324-1330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA sequencing and homologous expression of a small peptide conferring immunity to gassericin a, a circular bacteriocin a produced by lactobacillus gasseri LA39
AU - Kawai, Yasushi
AU - Kusnadi, Joni
AU - Kemperman, Rober
AU - Kok, Jan
AU - Ito, Yoshiyuki
AU - Endo, Mikiko
AU - Arakawa, Kensuke
AU - Uchida, Hideaki
AU - Nishimura, Junko
AU - Kitazawa, Haruki
AU - Saito, Tadao
PY - 2009/3
Y1 - 2009/3
N2 - Gassericin A, produced by Lactobacillus gasseri LA39, is a hydrophobic circular bacteriocin. The DNA region surrounding the gassericin A structural gene, gaaA, was sequenced, and seven open reading frames (ORFs) of 3.5 kbp (gaaBCADITE) were found with possible functions in gassericin A production, secretion, and immunity. The deduced products of the five consecutive ORFs gaaADITE have homology to those of genes involved in butyrivibriocin AR10 production, although the genetic arrangements are different in the two circular bacteriocin genes. Gaal is a small, positively charged hydrophobic peptide of 53 amino acids containing a putative transmembrane segment. Heterologous expression and homologous expression of Gaal in Lactococcus lactis subsp. cremoris MG1363 and L. gasseri JCM1131T, respectively, were studied. Gaal-expressing strains exhibited at least sevenfold-higher resistance to gassericin A than corresponding control strains, indicating that goal encodes an immunity peptide for gassericin A. Comparison of Gaal to peptides with similar characteristics found in the circular bacteriocin gene loci is discussed.
AB - Gassericin A, produced by Lactobacillus gasseri LA39, is a hydrophobic circular bacteriocin. The DNA region surrounding the gassericin A structural gene, gaaA, was sequenced, and seven open reading frames (ORFs) of 3.5 kbp (gaaBCADITE) were found with possible functions in gassericin A production, secretion, and immunity. The deduced products of the five consecutive ORFs gaaADITE have homology to those of genes involved in butyrivibriocin AR10 production, although the genetic arrangements are different in the two circular bacteriocin genes. Gaal is a small, positively charged hydrophobic peptide of 53 amino acids containing a putative transmembrane segment. Heterologous expression and homologous expression of Gaal in Lactococcus lactis subsp. cremoris MG1363 and L. gasseri JCM1131T, respectively, were studied. Gaal-expressing strains exhibited at least sevenfold-higher resistance to gassericin A than corresponding control strains, indicating that goal encodes an immunity peptide for gassericin A. Comparison of Gaal to peptides with similar characteristics found in the circular bacteriocin gene loci is discussed.
UR - http://www.scopus.com/inward/record.url?scp=61649115461&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61649115461&partnerID=8YFLogxK
U2 - 10.1128/AEM.02485-08
DO - 10.1128/AEM.02485-08
M3 - Article
C2 - 19114506
AN - SCOPUS:61649115461
VL - 75
SP - 1324
EP - 1330
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 5
ER -