DNA microarray analysis of submandibular glands in IgG4-related disease indicates a role for MARCO and other innate immune-related proteins

Miho Ohta, Masafumi Moriyama, Takashi Maehara, Yuka Gion, Sachiko Furukawa, Akihiko Tanaka, Jun Nosuke Hayashida, Masaki Yamauchi, Noriko Ishiguro, Yurie Mikami, Hiroto Tsuboi, Mana Iizuka-Koga, Shintaro Kawano, Yasuharu Sato, Tamotsu Kiyoshima, Takayuki Sumida, Seiji Nakamura

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Abstract

IgG4-related disease (IgG4-RD) is a novel systemic disease entity characterized by elevated serum IgG4 and tissue infiltration of IgG4-positive plasma cells accompanied by severe fibrosis. Although recent studies demonstrated that innate immune cells including monocytes and macrophages might promote local fibrosis and IgG4 production, the pathological mechanism remains unclear. In this study, we sought to identify the disease-associated genes, especially innate immune molecules. Gene expression was analyzed by DNA microarray in submandibular glands (SMGs) from patients with IgG4-RD (n=5), chronic sialoadenitis (CS) (n=3), and controls (n=3). Differentially expressed genes (DEGs) were validated by real-time polymerase chain reaction (PCR) and immunohistochemical staining in IgG4-RD (n=18), CS (n=4), Sjogren syndrome (n=11), and controls (n=10). Gene expression patterns in the 3 groups were quite different from each other by the pvclust method and principal components analysis. In IgG4-RD, 1028 upregulated genes and 692 downregulated genes were identified as DEGs (P

Original languageEnglish
Pages (from-to)e2853
JournalMedicine
Volume95
Issue number7
DOIs
Publication statusPublished - 2016

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Submandibular Gland
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Immunoglobulin G
Proteins
Sialadenitis
Genes
Fibrosis
Gene Expression
Sjogren's Syndrome
Plasma Cells
Principal Component Analysis
Real-Time Polymerase Chain Reaction
Monocytes
Down-Regulation
Macrophages
Staining and Labeling
Serum

ASJC Scopus subject areas

  • Medicine(all)

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DNA microarray analysis of submandibular glands in IgG4-related disease indicates a role for MARCO and other innate immune-related proteins. / Ohta, Miho; Moriyama, Masafumi; Maehara, Takashi; Gion, Yuka; Furukawa, Sachiko; Tanaka, Akihiko; Hayashida, Jun Nosuke; Yamauchi, Masaki; Ishiguro, Noriko; Mikami, Yurie; Tsuboi, Hiroto; Iizuka-Koga, Mana; Kawano, Shintaro; Sato, Yasuharu; Kiyoshima, Tamotsu; Sumida, Takayuki; Nakamura, Seiji.

In: Medicine, Vol. 95, No. 7, 2016, p. e2853.

Research output: Contribution to journalArticle

Ohta, M, Moriyama, M, Maehara, T, Gion, Y, Furukawa, S, Tanaka, A, Hayashida, JN, Yamauchi, M, Ishiguro, N, Mikami, Y, Tsuboi, H, Iizuka-Koga, M, Kawano, S, Sato, Y, Kiyoshima, T, Sumida, T & Nakamura, S 2016, 'DNA microarray analysis of submandibular glands in IgG4-related disease indicates a role for MARCO and other innate immune-related proteins', Medicine, vol. 95, no. 7, pp. e2853. https://doi.org/10.1097/MD.0000000000002853
Ohta, Miho ; Moriyama, Masafumi ; Maehara, Takashi ; Gion, Yuka ; Furukawa, Sachiko ; Tanaka, Akihiko ; Hayashida, Jun Nosuke ; Yamauchi, Masaki ; Ishiguro, Noriko ; Mikami, Yurie ; Tsuboi, Hiroto ; Iizuka-Koga, Mana ; Kawano, Shintaro ; Sato, Yasuharu ; Kiyoshima, Tamotsu ; Sumida, Takayuki ; Nakamura, Seiji. / DNA microarray analysis of submandibular glands in IgG4-related disease indicates a role for MARCO and other innate immune-related proteins. In: Medicine. 2016 ; Vol. 95, No. 7. pp. e2853.
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