DNA methylation errors in cloned mouse sperm by germ line barrier evasion

Tasuku Koike, Takuya Wakai, Yuko Jincho, Akihiko Sakashita, Hisato Kobayashi, Eiji Mizutani, Sayaka Wakayama, Fumihito Miura, Takashi Ito, Tomohiro Kono

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The germ line reprogramming barrier resets parental epigenetic modifications according to sex, conferring totipotency to mammalian embryos upon fertilization. However, it is not known whether epigenetic errors are committed during germ line reprogramming that are then transmitted to germ cells, and consequently to offspring. We addressed this question in the present study by performing a genome-wide DNA methylation analysis using a target postbisulfite sequencing method in order to identify DNA methylation errors in cloned mouse sperm. The sperm genomes of two somatic cell-cloned mice (CL1 and CL7) contained significantly higher numbers of differentially methylated CpG sites (P1/40.0045 and P1/40.0116). As a result, they had higher numbers of differentially methylated CpG islands. However, there was no evidence that these sites were transmitted to the sperm genome of offspring. These results suggest that DNA methylation errors resulting from embryo cloning are transmitted to the sperm genome by evading the germ line reprogramming barrier.

Original languageEnglish
Article number128
JournalBiology of Reproduction
Volume94
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Keywords

  • Cloning
  • DNA methylation
  • Epigenetics
  • Sperm
  • transgenerational effect

ASJC Scopus subject areas

  • Cell Biology

Cite this

Koike, T., Wakai, T., Jincho, Y., Sakashita, A., Kobayashi, H., Mizutani, E., Wakayama, S., Miura, F., Ito, T., & Kono, T. (2016). DNA methylation errors in cloned mouse sperm by germ line barrier evasion. Biology of Reproduction, 94(6), [128]. https://doi.org/10.1095/biolreprod.116.138677