DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation.

Y. Izumizawa, S. J. Yang, T. Negishi, K. Negishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sunlight is carcinogenic and mutagenic and its genotoxic effects are believed to be the result of UV light-induced lesions in DNA. These lesions include pyrimidine dimers and (6-4) photoproducts, but it is uncertain whether the pyrimidine modifications are the sole pre-mutagenic lesions induced by UV light. Previous studies indicate that some sunlight-induced mutations in the single-stranded DNA phage M13mp2 may not be caused by these photoproducts. In this work, purified single-stranded phage DNA was exposed to UVA, UVB and UVC and the induced mutations were analyzed. All 3 types of UV light increase the mutation frequency. The mutants were sequenced and the results suggest that UVA exposure may induce formation of a non-dipyrimidine lesion in DNA.

Original languageEnglish
Pages (from-to)73-74
Number of pages2
JournalNucleic acids symposium series
Issue number44
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Medicine(all)

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