DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation.

Y. Izumizawa, S. J. Yang, T. Negishi, K. Negishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sunlight is carcinogenic and mutagenic and its genotoxic effects are believed to be the result of UV light-induced lesions in DNA. These lesions include pyrimidine dimers and (6-4) photoproducts, but it is uncertain whether the pyrimidine modifications are the sole pre-mutagenic lesions induced by UV light. Previous studies indicate that some sunlight-induced mutations in the single-stranded DNA phage M13mp2 may not be caused by these photoproducts. In this work, purified single-stranded phage DNA was exposed to UVA, UVB and UVC and the induced mutations were analyzed. All 3 types of UV light increase the mutation frequency. The mutants were sequenced and the results suggest that UVA exposure may induce formation of a non-dipyrimidine lesion in DNA.

Original languageEnglish
Pages (from-to)73-74
Number of pages2
JournalNucleic acids symposium series
Issue number44
Publication statusPublished - 2000

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Ultraviolet Rays
Mutagenesis
Sunlight
Single-Stranded DNA
Bacteriophages
DNA
Pyrimidine Dimers
Mutation
Mutation Rate

Cite this

Izumizawa, Y., Yang, S. J., Negishi, T., & Negishi, K. (2000). DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation. Nucleic acids symposium series, (44), 73-74.

DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation. / Izumizawa, Y.; Yang, S. J.; Negishi, T.; Negishi, K.

In: Nucleic acids symposium series, No. 44, 2000, p. 73-74.

Research output: Contribution to journalArticle

Izumizawa, Y, Yang, SJ, Negishi, T & Negishi, K 2000, 'DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation.', Nucleic acids symposium series, no. 44, pp. 73-74.
Izumizawa, Y. ; Yang, S. J. ; Negishi, T. ; Negishi, K. / DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation. In: Nucleic acids symposium series. 2000 ; No. 44. pp. 73-74.
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