DNA copy number gains in malignant pleural mesothelioma

Masashi Furukawa, Shinichi Toyooka, Tatsuro Hayashi, Hiromasa Yamamoto, Nobukazu Fujimoto, Junichi Sou, Shinsuke Hashida, Kazuhiko Shien, Hiroaki Asano, Keisuke Aoe, Kazunori Okabe, Harvey I. Pass, Kazunori Tsukuda, Takumi Kishimoto, Shinichiro Miyoshi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Malignant pleural mesothelioma (MPM) is a highly aggressive tumor with an extremely poor prognosis. The incidence of MPM is increasing as a result of widespread exposure to asbestos. The molecular pathogenesis of MPM remains unclear. The present study analyzed the frequency of various genomic copy number gains (CNGs) in MPM using reverse transcription‑quantitative polymerase chain reaction. A total of 83 primary MPMs and 53 primary lung adenocarcinomas were analyzed to compare the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2. In MPM, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 12 (14.5%), 8 (9.6%), 5 (6.0%), 4 (4.8%) and 1 (1.2%) of the samples, respectively. In lung adenocarcinomas, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 21 (39.6%), 12 (22.6%), 5 (9.4%), 10 (18.9%) and 0 (0.0%) of the samples, respectively. The CNGs of EGFR, KRAS and FGFR1 were significantly less frequent in the MPMs compared with the lung adenocarcinomas (P=0.0018, 0.048 and 0.018, respectively). Overall, the MPMs exhibited these CNGs less frequently compared with the lung adenocarcinomas (P=0.0002). The differences in CNGs between the two tumor types suggested that they are genetically different.

Original languageEnglish
Pages (from-to)3274-3278
Number of pages5
JournalOncology Letters
Volume10
Issue number5
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

DNA
Asbestos
Neoplasms
Malignant Mesothelioma
Polymerase Chain Reaction
Adenocarcinoma of lung
Incidence

Keywords

  • Copy number
  • Lung adenocarcinoma
  • Malignant pleural mesothelioma
  • Reverse transcription-quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Furukawa, M., Toyooka, S., Hayashi, T., Yamamoto, H., Fujimoto, N., Sou, J., ... Miyoshi, S. (2015). DNA copy number gains in malignant pleural mesothelioma. Oncology Letters, 10(5), 3274-3278. https://doi.org/10.3892/ol.2015.3652

DNA copy number gains in malignant pleural mesothelioma. / Furukawa, Masashi; Toyooka, Shinichi; Hayashi, Tatsuro; Yamamoto, Hiromasa; Fujimoto, Nobukazu; Sou, Junichi; Hashida, Shinsuke; Shien, Kazuhiko; Asano, Hiroaki; Aoe, Keisuke; Okabe, Kazunori; Pass, Harvey I.; Tsukuda, Kazunori; Kishimoto, Takumi; Miyoshi, Shinichiro.

In: Oncology Letters, Vol. 10, No. 5, 01.11.2015, p. 3274-3278.

Research output: Contribution to journalArticle

Furukawa, M, Toyooka, S, Hayashi, T, Yamamoto, H, Fujimoto, N, Sou, J, Hashida, S, Shien, K, Asano, H, Aoe, K, Okabe, K, Pass, HI, Tsukuda, K, Kishimoto, T & Miyoshi, S 2015, 'DNA copy number gains in malignant pleural mesothelioma', Oncology Letters, vol. 10, no. 5, pp. 3274-3278. https://doi.org/10.3892/ol.2015.3652
Furukawa, Masashi ; Toyooka, Shinichi ; Hayashi, Tatsuro ; Yamamoto, Hiromasa ; Fujimoto, Nobukazu ; Sou, Junichi ; Hashida, Shinsuke ; Shien, Kazuhiko ; Asano, Hiroaki ; Aoe, Keisuke ; Okabe, Kazunori ; Pass, Harvey I. ; Tsukuda, Kazunori ; Kishimoto, Takumi ; Miyoshi, Shinichiro. / DNA copy number gains in malignant pleural mesothelioma. In: Oncology Letters. 2015 ; Vol. 10, No. 5. pp. 3274-3278.
@article{df1ef335fe2e44b582ef90425e9328ce,
title = "DNA copy number gains in malignant pleural mesothelioma",
abstract = "Malignant pleural mesothelioma (MPM) is a highly aggressive tumor with an extremely poor prognosis. The incidence of MPM is increasing as a result of widespread exposure to asbestos. The molecular pathogenesis of MPM remains unclear. The present study analyzed the frequency of various genomic copy number gains (CNGs) in MPM using reverse transcription‑quantitative polymerase chain reaction. A total of 83 primary MPMs and 53 primary lung adenocarcinomas were analyzed to compare the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2. In MPM, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 12 (14.5{\%}), 8 (9.6{\%}), 5 (6.0{\%}), 4 (4.8{\%}) and 1 (1.2{\%}) of the samples, respectively. In lung adenocarcinomas, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 21 (39.6{\%}), 12 (22.6{\%}), 5 (9.4{\%}), 10 (18.9{\%}) and 0 (0.0{\%}) of the samples, respectively. The CNGs of EGFR, KRAS and FGFR1 were significantly less frequent in the MPMs compared with the lung adenocarcinomas (P=0.0018, 0.048 and 0.018, respectively). Overall, the MPMs exhibited these CNGs less frequently compared with the lung adenocarcinomas (P=0.0002). The differences in CNGs between the two tumor types suggested that they are genetically different.",
keywords = "Copy number, Lung adenocarcinoma, Malignant pleural mesothelioma, Reverse transcription-quantitative polymerase chain reaction",
author = "Masashi Furukawa and Shinichi Toyooka and Tatsuro Hayashi and Hiromasa Yamamoto and Nobukazu Fujimoto and Junichi Sou and Shinsuke Hashida and Kazuhiko Shien and Hiroaki Asano and Keisuke Aoe and Kazunori Okabe and Pass, {Harvey I.} and Kazunori Tsukuda and Takumi Kishimoto and Shinichiro Miyoshi",
year = "2015",
month = "11",
day = "1",
doi = "10.3892/ol.2015.3652",
language = "English",
volume = "10",
pages = "3274--3278",
journal = "Oncology Letters",
issn = "1792-1074",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - DNA copy number gains in malignant pleural mesothelioma

AU - Furukawa, Masashi

AU - Toyooka, Shinichi

AU - Hayashi, Tatsuro

AU - Yamamoto, Hiromasa

AU - Fujimoto, Nobukazu

AU - Sou, Junichi

AU - Hashida, Shinsuke

AU - Shien, Kazuhiko

AU - Asano, Hiroaki

AU - Aoe, Keisuke

AU - Okabe, Kazunori

AU - Pass, Harvey I.

AU - Tsukuda, Kazunori

AU - Kishimoto, Takumi

AU - Miyoshi, Shinichiro

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Malignant pleural mesothelioma (MPM) is a highly aggressive tumor with an extremely poor prognosis. The incidence of MPM is increasing as a result of widespread exposure to asbestos. The molecular pathogenesis of MPM remains unclear. The present study analyzed the frequency of various genomic copy number gains (CNGs) in MPM using reverse transcription‑quantitative polymerase chain reaction. A total of 83 primary MPMs and 53 primary lung adenocarcinomas were analyzed to compare the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2. In MPM, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 12 (14.5%), 8 (9.6%), 5 (6.0%), 4 (4.8%) and 1 (1.2%) of the samples, respectively. In lung adenocarcinomas, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 21 (39.6%), 12 (22.6%), 5 (9.4%), 10 (18.9%) and 0 (0.0%) of the samples, respectively. The CNGs of EGFR, KRAS and FGFR1 were significantly less frequent in the MPMs compared with the lung adenocarcinomas (P=0.0018, 0.048 and 0.018, respectively). Overall, the MPMs exhibited these CNGs less frequently compared with the lung adenocarcinomas (P=0.0002). The differences in CNGs between the two tumor types suggested that they are genetically different.

AB - Malignant pleural mesothelioma (MPM) is a highly aggressive tumor with an extremely poor prognosis. The incidence of MPM is increasing as a result of widespread exposure to asbestos. The molecular pathogenesis of MPM remains unclear. The present study analyzed the frequency of various genomic copy number gains (CNGs) in MPM using reverse transcription‑quantitative polymerase chain reaction. A total of 83 primary MPMs and 53 primary lung adenocarcinomas were analyzed to compare the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2. In MPM, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 12 (14.5%), 8 (9.6%), 5 (6.0%), 4 (4.8%) and 1 (1.2%) of the samples, respectively. In lung adenocarcinomas, the CNGs of EGFR, KRAS, MET, FGFR1 and SOX2 were detected in 21 (39.6%), 12 (22.6%), 5 (9.4%), 10 (18.9%) and 0 (0.0%) of the samples, respectively. The CNGs of EGFR, KRAS and FGFR1 were significantly less frequent in the MPMs compared with the lung adenocarcinomas (P=0.0018, 0.048 and 0.018, respectively). Overall, the MPMs exhibited these CNGs less frequently compared with the lung adenocarcinomas (P=0.0002). The differences in CNGs between the two tumor types suggested that they are genetically different.

KW - Copy number

KW - Lung adenocarcinoma

KW - Malignant pleural mesothelioma

KW - Reverse transcription-quantitative polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=84942474895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942474895&partnerID=8YFLogxK

U2 - 10.3892/ol.2015.3652

DO - 10.3892/ol.2015.3652

M3 - Article

AN - SCOPUS:84942474895

VL - 10

SP - 3274

EP - 3278

JO - Oncology Letters

JF - Oncology Letters

SN - 1792-1074

IS - 5

ER -