DNA-binding properties of Mblk-1, a putative transcription factor from the honeybee

Jung Min Park, Takekazu Kunieda, Hideaki Takeuchi, Takeo Kubo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We previously identified a gene, Mblk-1, that encodes a putative transcription factor with two DNA-binding motifs expressed preferentially in the honeybee brain [H. Takeuchi et al., Insect Mol. Biol. 10, 487-494 (2001)]. In the present study, we identified its preferred binding sequence as 5′-CCCTATCGATCG-ATCTCTACCT-3′ and characterized its DNA-binding properties using truncated Mblk-1 mutants. An electrophoretic mobility shift assay revealed that the full-length Mblk-1 binds to the sequence with high affinity, whereas each truncated DNA-binding motif of Mblk-1 binds with much lower affinities. An in vitro pull-down assay indicated that each DNA-binding motif affords homodimeric bindings, suggesting that Mblk-1 functions as a dimer.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number1
DOIs
Publication statusPublished - Feb 15 2002
Externally publishedYes

Keywords

  • Brain
  • DNA binding
  • E93
  • Honeybee
  • Mblk-1
  • Mushroom body
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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