DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB

Gaku Mizuguchi, Hideki Nakagoshi, Takahiro Nagase, Nobuo Nomura, Takayasu Date, Yoshio Ueno, Shunsuke Ishii

Research output: Contribution to journalArticle

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Abstract

Three members of the myb gene family have been identified in human cDNA libraries c-myb, A-myb, and B-myb. We compared the DNA binding properties of the B-myb and c-myb proteins (B-MYB and c-MYB) using bacterially synthesized B-MYB and c-MYB in DNase I footprinting. B-MYB bound to most of the c-MYB binding sites examined, including the c-MYB binding site, MBS-I, in the simian virus (SV) 40 enhancer, in which the most frequent sequence was CCTAACTG. The MBS-I site was an enhancer element dependent on B-MYB and c-MYB in a co-transfection assay that used the B-myb or c-myb expression plasmid. Some sites in the SV40 genome, including the MBS-BI site, had high affinity with B-MYB but little or no affinity with c-MYB, in which the most frequent sequence was AGAAANPyrG. The MBS-BI site was an enhancer element dependent on B-MYB and a very weakly dependent on c-MYB. Our results showed that B-MYB is a transcriptional activator, like c-MYB, and that although B-MYB and c-MYB have similar sequence specificity for DNA binding some sequences were recognized by B-MYB preferentially.

Original languageEnglish
Pages (from-to)9280-9284
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number16
Publication statusPublished - Jun 5 1990
Externally publishedYes

Fingerprint

Activator Appliances
Proto-Oncogene Proteins c-myb
Genes
myb Genes
Binding Sites
Simian virus 40
Deoxyribonuclease I
DNA
Gene Library
Viruses
Transfection
Assays
Plasmids
Genome
human MYBL2 protein
IgA receptor

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mizuguchi, G., Nakagoshi, H., Nagase, T., Nomura, N., Date, T., Ueno, Y., & Ishii, S. (1990). DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB. Journal of Biological Chemistry, 265(16), 9280-9284.

DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB. / Mizuguchi, Gaku; Nakagoshi, Hideki; Nagase, Takahiro; Nomura, Nobuo; Date, Takayasu; Ueno, Yoshio; Ishii, Shunsuke.

In: Journal of Biological Chemistry, Vol. 265, No. 16, 05.06.1990, p. 9280-9284.

Research output: Contribution to journalArticle

Mizuguchi, G, Nakagoshi, H, Nagase, T, Nomura, N, Date, T, Ueno, Y & Ishii, S 1990, 'DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB', Journal of Biological Chemistry, vol. 265, no. 16, pp. 9280-9284.
Mizuguchi, Gaku ; Nakagoshi, Hideki ; Nagase, Takahiro ; Nomura, Nobuo ; Date, Takayasu ; Ueno, Yoshio ; Ishii, Shunsuke. / DNA binding activity and transcriptional activator function of the human B-myb protein compared with c-MYB. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 16. pp. 9280-9284.
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