Abstract
Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.
| Original language | English |
|---|---|
| Pages (from-to) | 75-77 |
| Number of pages | 3 |
| Journal | International Journal of Biological Macromolecules |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 1 1997 |
| Externally published | Yes |
Keywords
- Anti-DNA antibody
- DNA
- Enzyme-linked immunosorbent assay
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Molecular Biology
- Economics and Econometrics
- Energy(all)
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Cite this
Kitamura, H., Matsuura, E., Nagata, A., Sakairi, N., Tokura, S., & Nishi, N. (1997). DNA-alginate complex recognized by autoantibodies against DNA. International Journal of Biological Macromolecules, 20(1), 75-77. https://doi.org/10.1016/S0141-8130(97)01146-X