DNA-alginate complex recognized by autoantibodies against DNA

Hidemitsu Kitamura; Eiji Matsuura; Atsuo Nagata; Nobuo Sakairi; Seiichi Tokura; Norio Nishi

doi:10.1016/S0141-8130(97)01146-X

DNA-alginate complex recognized by autoantibodies against DNA

Hidemitsu Kitamura, Eiji Matsuura, Atsuo Nagata, Nobuo Sakairi, Seiichi Tokura, Norio Nishi

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.

Original language	English
Pages (from-to)	75-77
Number of pages	3
Journal	International Journal of Biological Macromolecules
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/S0141-8130(97)01146-X
Publication status	Published - Feb 1997
Externally published	Yes

Keywords

Anti-DNA antibody
DNA
Enzyme-linked immunosorbent assay

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology
Economics and Econometrics
Energy(all)

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10.1016/S0141-8130(97)01146-X

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@article{48105a1b02e641fdbde2582050778444,

title = "DNA-alginate complex recognized by autoantibodies against DNA",

abstract = "Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.",

keywords = "Anti-DNA antibody, DNA, Enzyme-linked immunosorbent assay",

author = "Hidemitsu Kitamura and Eiji Matsuura and Atsuo Nagata and Nobuo Sakairi and Seiichi Tokura and Norio Nishi",

note = "Funding Information: We thank Yuki Fine Chemical Co. Ltd., Japan for the kind gift of DNA. This work was supported by the Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (No. 07555600).",

year = "1997",

month = feb,

doi = "10.1016/S0141-8130(97)01146-X",

language = "English",

volume = "20",

pages = "75--77",

journal = "International Journal of Biological Macromolecules",

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TY - JOUR

T1 - DNA-alginate complex recognized by autoantibodies against DNA

AU - Kitamura, Hidemitsu

AU - Matsuura, Eiji

AU - Nagata, Atsuo

AU - Sakairi, Nobuo

AU - Tokura, Seiichi

AU - Nishi, Norio

N1 - Funding Information: We thank Yuki Fine Chemical Co. Ltd., Japan for the kind gift of DNA. This work was supported by the Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (No. 07555600).

PY - 1997/2

Y1 - 1997/2

N2 - Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.

AB - Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.

KW - Anti-DNA antibody

KW - DNA

KW - Enzyme-linked immunosorbent assay

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