Abstract
Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.
| Original language | English |
|---|---|
| Pages (from-to) | 75-77 |
| Number of pages | 3 |
| Journal | International Journal of Biological Macromolecules |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 1997 |
| Externally published | Yes |
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Keywords
- Anti-DNA antibody
- DNA
- Enzyme-linked immunosorbent assay
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
DNA-alginate complex recognized by autoantibodies against DNA. / Kitamura, Hidemitsu; Matsuura, Eiji; Nagata, Atsuo; Sakairi, Nobuo; Tokura, Seiichi; Nishi, Norio.
In: International Journal of Biological Macromolecules, Vol. 20, No. 1, 02.1997, p. 75-77.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA-alginate complex recognized by autoantibodies against DNA
AU - Kitamura, Hidemitsu
AU - Matsuura, Eiji
AU - Nagata, Atsuo
AU - Sakairi, Nobuo
AU - Tokura, Seiichi
AU - Nishi, Norio
PY - 1997/2
Y1 - 1997/2
N2 - Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.
AB - Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.
KW - Anti-DNA antibody
KW - DNA
KW - Enzyme-linked immunosorbent assay
UR - http://www.scopus.com/inward/record.url?scp=0030887239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030887239&partnerID=8YFLogxK
U2 - 10.1016/S0141-8130(97)01146-X
DO - 10.1016/S0141-8130(97)01146-X
M3 - Article
C2 - 9110187
AN - SCOPUS:0030887239
VL - 20
SP - 75
EP - 77
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
IS - 1
ER -