DNA-alginate complex recognized by autoantibodies against DNA

Hidemitsu Kitamura, Eiji Matsuura, Atsuo Nagata, Nobuo Sakairi, Seiichi Tokura, Norio Nishi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.

Original languageEnglish
Pages (from-to)75-77
Number of pages3
JournalInternational Journal of Biological Macromolecules
Volume20
Issue number1
DOIs
Publication statusPublished - Feb 1997
Externally publishedYes

Fingerprint

Autoantibodies
Immunosorbents
DNA
Assays
Enzyme-Linked Immunosorbent Assay
Antinuclear Antibodies
Polystyrenes
Enzymes
Lysine
Availability
Antigens
alginic acid
Antibodies

Keywords

  • Anti-DNA antibody
  • DNA
  • Enzyme-linked immunosorbent assay

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

DNA-alginate complex recognized by autoantibodies against DNA. / Kitamura, Hidemitsu; Matsuura, Eiji; Nagata, Atsuo; Sakairi, Nobuo; Tokura, Seiichi; Nishi, Norio.

In: International Journal of Biological Macromolecules, Vol. 20, No. 1, 02.1997, p. 75-77.

Research output: Contribution to journalArticle

Kitamura, Hidemitsu ; Matsuura, Eiji ; Nagata, Atsuo ; Sakairi, Nobuo ; Tokura, Seiichi ; Nishi, Norio. / DNA-alginate complex recognized by autoantibodies against DNA. In: International Journal of Biological Macromolecules. 1997 ; Vol. 20, No. 1. pp. 75-77.
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