DNA-alginate complex recognized by autoantibodies against DNA

Hidemitsu Kitamura, Eiji Matsuura, Atsuo Nagata, Nobuo Sakairi, Seiichi Tokura, Norio Nishi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected ratheer than to poly-L-lysine. These results show an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.

Original languageEnglish
Pages (from-to)75-77
Number of pages3
JournalInternational Journal of Biological Macromolecules
Volume20
Issue number1
DOIs
Publication statusPublished - Feb 1 1997
Externally publishedYes

Keywords

  • Anti-DNA antibody
  • DNA
  • Enzyme-linked immunosorbent assay

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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