DkMyb2 wound-induced transcription factor of persimmon (Diospyros kaki Thunb.), contributes to proanthocyanidin regulation

Takashi Akagi, Ayako Ikegami, Keizo Yonemori

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Proanthocyanidins (PAs) are secondary metabolites that contribute to the protection of a plant against biotic and abiotic stresses. Persimmon (Diospyros kaki) accumulates abundant PAs in each plant organ, and some potential Myb-like transcription factors (Myb-TFs) involved in the production of PAs have been isolated. In this study, we aimed to molecularly characterize one of them, DkMyb2, which was placed in a subclade including a PA regulator of Arabidopsis (Arabidopsis thaliana), TRANSPARENT TESTA2 (TT2), and was co-induced with PA pathway genes after wound stress. Ectopic DkMyb2 overexpression caused significant up-regulation of PA pathway genes in transgenic persimmon calluses and significant accumulation of PA, and increased mean degree of polymerization of PAs in transgenic kiwifruit calluses. Analysis of the DNA-binding ability of DkMyb2 by electrophoretic mobility shift assays showed that DkMyb2 directly binds to the AC-rich cis-motifs known as AC elements in the promoters of the two PA pathway genes in persimmon, DkANR, and DkLAR. Furthermore, a transient reporter assay using a dual-luciferase system demonstrated direct transcriptional activation of DkANR and DkLAR by DkMyb2. We also discuss subfunctionalization of two PA regulators in persimmon, DkMyb2 and DkMyb4, as well as PA regulators in other plant species from the viewpoint of their ability to bind to cis-motifs and their functions in transcriptional activation. Our results provide insight into the multiple regulatory mechanisms that control PA metabolism by Myb-TFs in persimmon.

Original languageEnglish
Pages (from-to)1045-1059
Number of pages15
JournalPlanta
Volume232
Issue number5
DOIs
Publication statusPublished - Aug 6 2010
Externally publishedYes

Fingerprint

Diospyros
Diospyros kaki
Proanthocyanidins
persimmons
plant damage
proanthocyanidins
Transcription Factors
transcription factors
Wounds and Injuries
Bony Callus
transcriptional activation
Arabidopsis
Transcriptional Activation
proanthocyanidin
callus
genetically modified organisms
Genes
genes
binding capacity
kiwifruit

Keywords

  • Cis-motif
  • DkMyb2
  • DkMyb4
  • Persimmon
  • Proanthocyanidin

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

DkMyb2 wound-induced transcription factor of persimmon (Diospyros kaki Thunb.), contributes to proanthocyanidin regulation. / Akagi, Takashi; Ikegami, Ayako; Yonemori, Keizo.

In: Planta, Vol. 232, No. 5, 06.08.2010, p. 1045-1059.

Research output: Contribution to journalArticle

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