Diversity of immunobiological functions of T-cell lines established from patients with adult T-cell leukaemia

K. Iwatsuki, H. Harada, Y. Motoki, F. Kaneko, F. Jin, M. Takigawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-) cells secreted elevated quantities of IL-4, IL-6 and IFN-γ, and expressed mRNA for each cytokine in the absence of exogenous stimulation. The 35T(-) cells secreted IL-6 and a small amount of IFN-γ, but not IL-4. The MH-1 and KS-2 cells secreted only IL-6 in the absence of stimulation. In response to stimulation with phorbol-12-myristate-13 acetate (PMA), the 16T(-) cells produced more IL-4 and IFN-γ, whereas the 35T(-) and MH-1 cells exhibited increased secretion of IFN-γ, but still no IL-4 or IL-4 mRNA production. Although neither IL-4 nor IFN-γ were found in the culture supernatant of KS-2 cells, the production of IL-4 mRNA was detected by RT-PCR. Culture supernatants from the 16T(-) and 35T(-) cells induced the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-DR by cultured keratinocytes. This response was inhibited by pretreatment of the supernatant with anti-IFN-γ antibodies. These results indicate that some HTLV-1-infected T-cell lines constitutively secrete various cytokines, including biologically active IFN-γ. The diversity of immunobiological functions of the T-cell lines may be related to the variety of clinical features present in ATL patients.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalBritish Journal of Dermatology
Volume133
Issue number6
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Adult T Cell Leukemia Lymphoma
Interleukin-4
T-Lymphocytes
Cell Line
Human T-lymphotropic virus 1
Interleukin-6
Messenger RNA
Cytokines
HLA-DR Antigens
Intercellular Adhesion Molecule-1
Keratinocytes
Skin Diseases
Anti-Idiotypic Antibodies
Acetates
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Dermatology

Cite this

Diversity of immunobiological functions of T-cell lines established from patients with adult T-cell leukaemia. / Iwatsuki, K.; Harada, H.; Motoki, Y.; Kaneko, F.; Jin, F.; Takigawa, M.

In: British Journal of Dermatology, Vol. 133, No. 6, 1995, p. 861-867.

Research output: Contribution to journalArticle

Iwatsuki, K. ; Harada, H. ; Motoki, Y. ; Kaneko, F. ; Jin, F. ; Takigawa, M. / Diversity of immunobiological functions of T-cell lines established from patients with adult T-cell leukaemia. In: British Journal of Dermatology. 1995 ; Vol. 133, No. 6. pp. 861-867.
@article{df65676d46b94413a7e30bbf059d1cb0,
title = "Diversity of immunobiological functions of T-cell lines established from patients with adult T-cell leukaemia",
abstract = "In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-) cells secreted elevated quantities of IL-4, IL-6 and IFN-γ, and expressed mRNA for each cytokine in the absence of exogenous stimulation. The 35T(-) cells secreted IL-6 and a small amount of IFN-γ, but not IL-4. The MH-1 and KS-2 cells secreted only IL-6 in the absence of stimulation. In response to stimulation with phorbol-12-myristate-13 acetate (PMA), the 16T(-) cells produced more IL-4 and IFN-γ, whereas the 35T(-) and MH-1 cells exhibited increased secretion of IFN-γ, but still no IL-4 or IL-4 mRNA production. Although neither IL-4 nor IFN-γ were found in the culture supernatant of KS-2 cells, the production of IL-4 mRNA was detected by RT-PCR. Culture supernatants from the 16T(-) and 35T(-) cells induced the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-DR by cultured keratinocytes. This response was inhibited by pretreatment of the supernatant with anti-IFN-γ antibodies. These results indicate that some HTLV-1-infected T-cell lines constitutively secrete various cytokines, including biologically active IFN-γ. The diversity of immunobiological functions of the T-cell lines may be related to the variety of clinical features present in ATL patients.",
author = "K. Iwatsuki and H. Harada and Y. Motoki and F. Kaneko and F. Jin and M. Takigawa",
year = "1995",
doi = "10.1111/j.1365-2133.1995.tb06917.x",
language = "English",
volume = "133",
pages = "861--867",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Diversity of immunobiological functions of T-cell lines established from patients with adult T-cell leukaemia

AU - Iwatsuki, K.

AU - Harada, H.

AU - Motoki, Y.

AU - Kaneko, F.

AU - Jin, F.

AU - Takigawa, M.

PY - 1995

Y1 - 1995

N2 - In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-) cells secreted elevated quantities of IL-4, IL-6 and IFN-γ, and expressed mRNA for each cytokine in the absence of exogenous stimulation. The 35T(-) cells secreted IL-6 and a small amount of IFN-γ, but not IL-4. The MH-1 and KS-2 cells secreted only IL-6 in the absence of stimulation. In response to stimulation with phorbol-12-myristate-13 acetate (PMA), the 16T(-) cells produced more IL-4 and IFN-γ, whereas the 35T(-) and MH-1 cells exhibited increased secretion of IFN-γ, but still no IL-4 or IL-4 mRNA production. Although neither IL-4 nor IFN-γ were found in the culture supernatant of KS-2 cells, the production of IL-4 mRNA was detected by RT-PCR. Culture supernatants from the 16T(-) and 35T(-) cells induced the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-DR by cultured keratinocytes. This response was inhibited by pretreatment of the supernatant with anti-IFN-γ antibodies. These results indicate that some HTLV-1-infected T-cell lines constitutively secrete various cytokines, including biologically active IFN-γ. The diversity of immunobiological functions of the T-cell lines may be related to the variety of clinical features present in ATL patients.

AB - In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-) cells secreted elevated quantities of IL-4, IL-6 and IFN-γ, and expressed mRNA for each cytokine in the absence of exogenous stimulation. The 35T(-) cells secreted IL-6 and a small amount of IFN-γ, but not IL-4. The MH-1 and KS-2 cells secreted only IL-6 in the absence of stimulation. In response to stimulation with phorbol-12-myristate-13 acetate (PMA), the 16T(-) cells produced more IL-4 and IFN-γ, whereas the 35T(-) and MH-1 cells exhibited increased secretion of IFN-γ, but still no IL-4 or IL-4 mRNA production. Although neither IL-4 nor IFN-γ were found in the culture supernatant of KS-2 cells, the production of IL-4 mRNA was detected by RT-PCR. Culture supernatants from the 16T(-) and 35T(-) cells induced the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-DR by cultured keratinocytes. This response was inhibited by pretreatment of the supernatant with anti-IFN-γ antibodies. These results indicate that some HTLV-1-infected T-cell lines constitutively secrete various cytokines, including biologically active IFN-γ. The diversity of immunobiological functions of the T-cell lines may be related to the variety of clinical features present in ATL patients.

UR - http://www.scopus.com/inward/record.url?scp=0029561108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029561108&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2133.1995.tb06917.x

DO - 10.1111/j.1365-2133.1995.tb06917.x

M3 - Article

C2 - 8547036

AN - SCOPUS:0029561108

VL - 133

SP - 861

EP - 867

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 6

ER -