Diversity in photosynthetic electron transport under [CO2]-limitation: the cyanobacterium Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O2-dependent alternative electron flow and non-photochemical quenching of chlorophyll fluorescence during CO2-limited photosynthesis

Ginga Shimakawa, Seiji Akimoto, Yoshifumi Ueno, Ayumi Wada, Keiichiro Shaku, Yuichiro Takahashi, Chikahiro Miyake

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Some cyanobacteria, but not all, experience an induction of alternative electron flow (AEF) during CO2-limited photosynthesis. For example, Synechocystis sp. PCC 6803 (S. 6803) exhibits AEF, but Synechococcus elongatus sp. PCC 7942 does not. This difference is due to the presence of flavodiiron 2 and 4 proteins (FLV2/4) in S. 6803, which catalyze electron donation to O2. In this study, we observed a low-[CO2] induced AEF in the marine cyanobacterium Synechococcus sp. PCC 7002 that lacks FLV2/4. The AEF shows high affinity for O2, compared with AEF mediated by FLV2/4 in S. 6803, and can proceed under extreme low [O2] (about a few µM O2). Further, the transition from CO2-saturated to CO2-limited photosynthesis leads a preferential excitation of PSI to PSII and increased non-photochemical quenching of chlorophyll fluorescence. We found that the model green alga Chlamydomonas reinhardtii also has an O2-dependent AEF showing the same affinity for O2 as that in S. 7002. These data represent the diverse molecular mechanisms to drive AEF in cyanobacteria and green algae. In this paper, we further discuss the diversity, the evolution, and the physiological function of strategy to CO2-limitation in cyanobacterial and green algal photosynthesis.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalPhotosynthesis Research
DOIs
Publication statusAccepted/In press - Mar 29 2016

Fingerprint

Synechococcus sp. PCC 7002
Synechococcus
Chlamydomonas reinhardtii
Chlorophyta
Photosynthesis
Cyanobacteria
Chlorophyll
Algae
Electron Transport
electron transfer
Quenching
Fluorescence
electrons
photosynthesis
fluorescence
Electrons
chlorophyll
Protein S
Synechococcus elongatus
Synechocystis sp. PCC 6803

Keywords

  • Alternative electron flow
  • Non-photochemical quenching
  • Photosynthetic electron transport
  • Species diversity

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

@article{35a4d483d858435b87c2b75f71ae3668,
title = "Diversity in photosynthetic electron transport under [CO2]-limitation: the cyanobacterium Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O2-dependent alternative electron flow and non-photochemical quenching of chlorophyll fluorescence during CO2-limited photosynthesis",
abstract = "Some cyanobacteria, but not all, experience an induction of alternative electron flow (AEF) during CO2-limited photosynthesis. For example, Synechocystis sp. PCC 6803 (S. 6803) exhibits AEF, but Synechococcus elongatus sp. PCC 7942 does not. This difference is due to the presence of flavodiiron 2 and 4 proteins (FLV2/4) in S. 6803, which catalyze electron donation to O2. In this study, we observed a low-[CO2] induced AEF in the marine cyanobacterium Synechococcus sp. PCC 7002 that lacks FLV2/4. The AEF shows high affinity for O2, compared with AEF mediated by FLV2/4 in S. 6803, and can proceed under extreme low [O2] (about a few µM O2). Further, the transition from CO2-saturated to CO2-limited photosynthesis leads a preferential excitation of PSI to PSII and increased non-photochemical quenching of chlorophyll fluorescence. We found that the model green alga Chlamydomonas reinhardtii also has an O2-dependent AEF showing the same affinity for O2 as that in S. 7002. These data represent the diverse molecular mechanisms to drive AEF in cyanobacteria and green algae. In this paper, we further discuss the diversity, the evolution, and the physiological function of strategy to CO2-limitation in cyanobacterial and green algal photosynthesis.",
keywords = "Alternative electron flow, Non-photochemical quenching, Photosynthetic electron transport, Species diversity",
author = "Ginga Shimakawa and Seiji Akimoto and Yoshifumi Ueno and Ayumi Wada and Keiichiro Shaku and Yuichiro Takahashi and Chikahiro Miyake",
year = "2016",
month = "3",
day = "29",
doi = "10.1007/s11120-016-0253-y",
language = "English",
pages = "1--13",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Diversity in photosynthetic electron transport under [CO2]-limitation

T2 - the cyanobacterium Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O2-dependent alternative electron flow and non-photochemical quenching of chlorophyll fluorescence during CO2-limited photosynthesis

AU - Shimakawa, Ginga

AU - Akimoto, Seiji

AU - Ueno, Yoshifumi

AU - Wada, Ayumi

AU - Shaku, Keiichiro

AU - Takahashi, Yuichiro

AU - Miyake, Chikahiro

PY - 2016/3/29

Y1 - 2016/3/29

N2 - Some cyanobacteria, but not all, experience an induction of alternative electron flow (AEF) during CO2-limited photosynthesis. For example, Synechocystis sp. PCC 6803 (S. 6803) exhibits AEF, but Synechococcus elongatus sp. PCC 7942 does not. This difference is due to the presence of flavodiiron 2 and 4 proteins (FLV2/4) in S. 6803, which catalyze electron donation to O2. In this study, we observed a low-[CO2] induced AEF in the marine cyanobacterium Synechococcus sp. PCC 7002 that lacks FLV2/4. The AEF shows high affinity for O2, compared with AEF mediated by FLV2/4 in S. 6803, and can proceed under extreme low [O2] (about a few µM O2). Further, the transition from CO2-saturated to CO2-limited photosynthesis leads a preferential excitation of PSI to PSII and increased non-photochemical quenching of chlorophyll fluorescence. We found that the model green alga Chlamydomonas reinhardtii also has an O2-dependent AEF showing the same affinity for O2 as that in S. 7002. These data represent the diverse molecular mechanisms to drive AEF in cyanobacteria and green algae. In this paper, we further discuss the diversity, the evolution, and the physiological function of strategy to CO2-limitation in cyanobacterial and green algal photosynthesis.

AB - Some cyanobacteria, but not all, experience an induction of alternative electron flow (AEF) during CO2-limited photosynthesis. For example, Synechocystis sp. PCC 6803 (S. 6803) exhibits AEF, but Synechococcus elongatus sp. PCC 7942 does not. This difference is due to the presence of flavodiiron 2 and 4 proteins (FLV2/4) in S. 6803, which catalyze electron donation to O2. In this study, we observed a low-[CO2] induced AEF in the marine cyanobacterium Synechococcus sp. PCC 7002 that lacks FLV2/4. The AEF shows high affinity for O2, compared with AEF mediated by FLV2/4 in S. 6803, and can proceed under extreme low [O2] (about a few µM O2). Further, the transition from CO2-saturated to CO2-limited photosynthesis leads a preferential excitation of PSI to PSII and increased non-photochemical quenching of chlorophyll fluorescence. We found that the model green alga Chlamydomonas reinhardtii also has an O2-dependent AEF showing the same affinity for O2 as that in S. 7002. These data represent the diverse molecular mechanisms to drive AEF in cyanobacteria and green algae. In this paper, we further discuss the diversity, the evolution, and the physiological function of strategy to CO2-limitation in cyanobacterial and green algal photosynthesis.

KW - Alternative electron flow

KW - Non-photochemical quenching

KW - Photosynthetic electron transport

KW - Species diversity

UR - http://www.scopus.com/inward/record.url?scp=84962301166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962301166&partnerID=8YFLogxK

U2 - 10.1007/s11120-016-0253-y

DO - 10.1007/s11120-016-0253-y

M3 - Article

C2 - 27026083

AN - SCOPUS:84962301166

SP - 1

EP - 13

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

ER -